Matrix modifying reagent for detecting content of lead and cadmium elements in whole blood and application thereof

A technology of matrix improvement and element content, applied in the measurement of color/spectral characteristics, etc., can solve the problems of high requirements for personnel quality, complicated operation steps, expensive instruments, etc., and achieve convenient operation methods, reduce pain, and take less blood. Effect

Active Publication Date: 2009-12-02
BEIJING BOHUI INNOVATION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patent can complete the measurement of lead and cadmium in the sample at the same time, which shortens the detection time and improves the measurement accuracy. If it is within the linear range of the calibration curve, it is necessary to modify the matrix of the whole blood. The traditional atomic analysis method takes a large amount of blood, generally above 2mL, which is likely to cause great pain to children and other groups. Although the method of microwave sample dissolution can be used Do sample pretreatment to reduce the amount of blood taken, but the equipment is expensive, the operation steps are cumbersome and there are certain risks, and the requirements for the environment and personnel quality of testing institutions such as hospitals are relatively high

Method used

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  • Matrix modifying reagent for detecting content of lead and cadmium elements in whole blood and application thereof
  • Matrix modifying reagent for detecting content of lead and cadmium elements in whole blood and application thereof
  • Matrix modifying reagent for detecting content of lead and cadmium elements in whole blood and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Matrix-improving reagents and methods for their preparation and use:

[0018] The matrix improvement reagent formula is as follows: diammonium hydrogen phosphate 5‰, polyethylene glycol octylphenyl ether 2.0‰, concentrated nitric acid 1.0‰, and the rest is ultrapure water.

[0019] Preparation: Weigh 10.00 grams of high-grade pure diammonium hydrogen phosphate, put it in a 2000mL volumetric flask, add 4.0mL polyethylene glycol octylphenyl ether, 2.0mL concentrated nitric acid, then dilute to the mark with ultrapure water, press The specifications are divided into centrifuge tubes to obtain the finished product.

[0020] The method for detecting lead and cadmium element content in whole blood with it comprises the following steps:

[0021] (1) Take a centrifuge tube containing 0.07mL matrix improvement reagent and set aside;

[0022] (2) At 10°C-30°C, add 10 μL of whole blood to the centrifuge tube containing the matrix improvement reagent, cover the centrifuge tube ti...

Embodiment 2

[0025] Matrix-improving reagents and methods for their preparation and use:

[0026] The matrix improvement reagent formula is as follows: diammonium hydrogen phosphate 5.5‰, polyethylene glycol octylphenyl ether 3.0‰, concentrated nitric acid 2.0‰, and the rest is ultrapure water.

[0027] The preparation method is the same as in Example 1.

[0028] The method for detecting lead and cadmium element content in whole blood with it comprises the following steps:

[0029] (1) Take a centrifuge tube containing 0.09mL matrix improvement reagent and set it aside;

[0030] (2) At 10°C-30°C, add 10 μL of whole blood to the centrifuge tube containing the matrix improvement reagent, close the cap of the centrifuge tube, shake and mix quickly to dilute the whole blood to one-tenth of the original concentration , standing still for inspection;

[0031] (3) On the BH2100S / T atomic absorption spectrometer (where S means plastic shell and T means iron shell) produced by Beijing Bohui Inno...

Embodiment 3

[0033] Matrix-improving reagents and methods for their preparation and use:

[0034] The matrix improvement reagent formula is as follows: diammonium hydrogen phosphate 6‰, polyethylene glycol octylphenyl ether 4.0‰, concentrated nitric acid 3.0‰, and the rest is ultrapure water.

[0035] The preparation method is the same as in Example 1.

[0036] The method for detecting lead and cadmium element content in whole blood with it comprises the following steps:

[0037] (1) Take a centrifuge tube containing 0.12mL matrix improvement reagent and set aside;

[0038] (2) At 10°C-30°C, add 10 μL of whole blood to the centrifuge tube containing the matrix improvement reagent, close the cap of the centrifuge tube, shake and mix quickly to dilute the whole blood to 1 / 13 of the original concentration 1. Stand still and wait for inspection;

[0039] (3) Detection by BH2100S / T atomic absorption spectrometer produced by Beijing Bohui Innovation Optoelectronics Technology Co., Ltd.

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Abstract

The invention discloses a matrix modifying reagent for detecting content of lead and cadmium elements in whole blood, which comprises the following compositions: diammonium phosphate with the mass concentration of 0.5 to 0.6 percent, millesimal polyoxyethylene octyl phenyl ether with volume concentration of 2.0 to 4.0 percent, millesimal concentrated nitric acid with volume concentration of 1.0 to 3.0, and the balance of ultrapure water. The invention also provides a method for detecting content of lead and cadmium elements in the whole blood by the matrix modifying reagent, which dilutes concentration of the whole blood to be between one eighth and one thirteenth of the original concentration by adopting the matrix modifying reagent, and adds the diluted blood to an atomic absorption spectrometer for detecting. The detection method can detect lead and cadmium elements in one time, and has small amount of blood sample, simple operation, and low requirement on environment and operators.

Description

technical field [0001] The invention relates to a matrix-improved reagent and a detection method, in particular to a matrix-improved reagent for detecting the content of lead and cadmium elements in whole blood and a method for detecting the content of lead and cadmium elements in whole blood by using the matrix-improved reagent. Background technique [0002] In recent years, with the improvement of my country's industrial level, the types and quantities of toxic or potentially toxic chemicals in the environment, especially heavy metals, have increased, posing a serious threat to humans and animals, among which the hazards of cadmium and lead are particularly prominent. Cadmium is a by-product of zinc extraction, which is mostly used in the fields of electroplating and alloy manufacturing. Studies have shown that cadmium poisoning can cause renal tubular reabsorption disorders, resulting in the loss of low molecular weight proteins and calcium in the urine, which will easily ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
Inventor 杨奇
Owner BEIJING BOHUI INNOVATION TECH
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