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Whole blood matrix modifying reagent and method for detecting multielement content in whole blood thereof

A technology of matrix improvement and reagents, which is applied in the field of detection of multi-element content in whole blood, can solve the problems of pretreatment liquid ratio error, long time-consuming instrument measurement process, difficulty in popularization and use, etc., so as to prevent sample contamination and benefit The effect of promotional use and convenient operation

Active Publication Date: 2013-01-09
BEIJING BOHUI INNOVATION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the sample pretreatment process, firstly, in the transfer, evaporation and atomization process of the sample, the slight difference in the viscosity, surface tension and density of the sample solution will cause changes in the spray speed, aerosol size and transmission efficiency. , which in turn causes changes in the absorption intensity and affects the measurement accuracy
In the second aspect, due to the small amount of detection, the proportion accuracy of the pretreatment solution is slightly wrong, which will directly affect the measurement results. Therefore, suitable matrix improvement reagents and strict consistency proportions are required.
[0005] In the process of instrument measurement, because traditional instruments can only detect a single element, and the instrument parameters must be readjusted for each element detected, not only multiple detections are required, but also the steps are cumbersome, time-consuming, and detection efficiency is low
[0006] Therefore, in summary, in the field of clinical testing, due to factors such as large blood volume, cumbersome sample pretreatment steps, high requirements, long time-consuming instrument measurement, and low detection efficiency, atomic absorption spectrometry is used to detect trace amounts of blood in blood. The method of elements is difficult to popularize and promote

Method used

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  • Whole blood matrix modifying reagent and method for detecting multielement content in whole blood thereof
  • Whole blood matrix modifying reagent and method for detecting multielement content in whole blood thereof
  • Whole blood matrix modifying reagent and method for detecting multielement content in whole blood thereof

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Effect test

Embodiment 1

[0024] Whole blood matrix improvement reagent and its preparation and use method:

[0025] Whole blood matrix improvement reagent formula: lanthanum chloride 3‰, polyethylene glycol octylphenyl ether 0.5‰, concentrated nitric acid 0.5‰, and the rest is ultrapure water.

[0026] The preparation method comprises the following steps:

[0027] (1), weigh 200.00 grams of high-grade pure lanthanum chloride, place it in a 1000mL volumetric flask, add ultrapure water to dissolve, set the volume to the mark, and set aside;

[0028] (2) Take 10mL of high-grade pure polyethylene glycol octylphenyl ether, transfer it into a 100mL volumetric flask, add ultrapure water to dissolve, set the volume to the mark, and set aside;

[0029] (3), get the lanthanum chloride solution 30mL that step (1) makes, add in the volumetric flask of 2000mL respectively with the polyethylene glycol octyl phenyl ether solution 10mL that step (2) makes, concentrated nitric acid 1.0mL, Add ultrapure water to make...

Embodiment 2

[0035] Whole blood matrix improvement reagent and its preparation and use method:

[0036] Formula: lanthanum chloride 5‰, polyethylene glycol octylphenyl ether 1‰, concentrated nitric acid 0.75‰, and the rest is ultrapure water.

[0037] The preparation method is the same as in Example 1.

[0038] Using it to detect multiple elements in whole blood:

[0039] (1) Take a centrifuge tube containing 0.30mL whole blood matrix improvement reagent and set it aside;

[0040] (2) At 10-30°C, add 10 μL of whole blood to the centrifuge tube containing the whole blood matrix improvement reagent, cover the centrifuge tube tightly, shake and mix quickly, and dilute the whole blood to 31 minutes of the original concentration. One of them is still waiting for inspection;

[0041] (3) On-machine detection, the instrument used is the BH7100S / T atomic absorption spectrometer produced by Beijing Bohui Innovation Optoelectronics Technology Co., Ltd.

Embodiment 3

[0043] Whole blood matrix improvement reagent and its preparation and use method:

[0044] Formula: lanthanum chloride 7‰, polyethylene glycol octylphenyl ether 1.5‰, concentrated nitric acid 1.5‰, and the rest is ultrapure water.

[0045] The preparation method is the same as in Example 1.

[0046] Using it to detect multiple elements in whole blood:

[0047] (1) Take a centrifuge tube containing 0.34mL whole blood matrix improvement reagent and set aside;

[0048] (2) At 10-30°C, add 10 μL of whole blood to the centrifuge tube containing the whole blood matrix improvement reagent, cover the centrifuge tube tightly, shake and mix quickly, and dilute the whole blood to 35 minutes of the original concentration. One of them is still waiting for inspection;

[0049] (3) On-board detection, the instrument used is the BH5300S atomic absorption spectrometer produced by Beijing Bohui Innovation Optoelectronics Technology Co., Ltd.

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Abstract

The invention discloses a whole blood matrix modifying reagent. The modifying reagent comprises the following compositions: 3 to 7 mass millesimal lanthanum chloride, 0.5 to 1.5 volume millesimal polyoxyethylene octyl phenyl ether, 0.5 to 1.5 volume millesimal concentrated nitric acid, and the balance of ultrapure water. The invention also provides a method for simultaneously detecting multielement by the whole blood matrix modifying reagent, which dilutes concentration of the whole blood to be between one twenty-sixth and one thirty-fifth of the original concentration by adopting the whole blood matrix modifying reagent, and installs a multielement simultaneous measuring atomic absorption device for detecting after standing the whole blood. The method can simultaneously detect multiple trace elements in the whole blood, and has good environmental applicability, high measuring accuracy and simple operation.

Description

technical field [0001] The invention relates to a matrix-improved reagent and a detection method, in particular to a whole blood matrix-improved reagent for simultaneous detection of multi-element content in whole blood and a method for using it to detect multi-element content in whole blood. Background technique [0002] Atomic absorption spectrophotometry is a modern instrumental analysis method based on the characteristic radiation of the measured element emitted from the light source being absorbed by its ground state atoms when it passes through the atomic vapor of the measured sample, and the content in the measured sample is determined by the degree of radiation attenuation, because With the advantages of strong selectivity, less interference, high accuracy, and fast analysis speed, it has become one of the classic spectral detection methods for trace and trace elements. In the detection of trace elements in biological materials, the most common clinical method is to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/50G01N21/31G01N1/28
Inventor 杨奇
Owner BEIJING BOHUI INNOVATION TECH
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