Whole blood matrix modifying reagent and method for detecting multielement content in whole blood thereof
A technology of matrix improvement and reagents, which is applied in the field of detection of multi-element content in whole blood, can solve the problems of pretreatment liquid ratio error, long time-consuming instrument measurement process, difficulty in popularization and use, etc., so as to prevent sample contamination and benefit The effect of promotional use and convenient operation
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Embodiment 1
[0024] Whole blood matrix improvement reagent and its preparation and use method:
[0025] Whole blood matrix improvement reagent formula: lanthanum chloride 3‰, polyethylene glycol octylphenyl ether 0.5‰, concentrated nitric acid 0.5‰, and the rest is ultrapure water.
[0026] The preparation method comprises the following steps:
[0027] (1), weigh 200.00 grams of high-grade pure lanthanum chloride, place it in a 1000mL volumetric flask, add ultrapure water to dissolve, set the volume to the mark, and set aside;
[0028] (2) Take 10mL of high-grade pure polyethylene glycol octylphenyl ether, transfer it into a 100mL volumetric flask, add ultrapure water to dissolve, set the volume to the mark, and set aside;
[0029] (3), get the lanthanum chloride solution 30mL that step (1) makes, add in the volumetric flask of 2000mL respectively with the polyethylene glycol octyl phenyl ether solution 10mL that step (2) makes, concentrated nitric acid 1.0mL, Add ultrapure water to make...
Embodiment 2
[0035] Whole blood matrix improvement reagent and its preparation and use method:
[0036] Formula: lanthanum chloride 5‰, polyethylene glycol octylphenyl ether 1‰, concentrated nitric acid 0.75‰, and the rest is ultrapure water.
[0037] The preparation method is the same as in Example 1.
[0038] Using it to detect multiple elements in whole blood:
[0039] (1) Take a centrifuge tube containing 0.30mL whole blood matrix improvement reagent and set it aside;
[0040] (2) At 10-30°C, add 10 μL of whole blood to the centrifuge tube containing the whole blood matrix improvement reagent, cover the centrifuge tube tightly, shake and mix quickly, and dilute the whole blood to 31 minutes of the original concentration. One of them is still waiting for inspection;
[0041] (3) On-machine detection, the instrument used is the BH7100S / T atomic absorption spectrometer produced by Beijing Bohui Innovation Optoelectronics Technology Co., Ltd.
Embodiment 3
[0043] Whole blood matrix improvement reagent and its preparation and use method:
[0044] Formula: lanthanum chloride 7‰, polyethylene glycol octylphenyl ether 1.5‰, concentrated nitric acid 1.5‰, and the rest is ultrapure water.
[0045] The preparation method is the same as in Example 1.
[0046] Using it to detect multiple elements in whole blood:
[0047] (1) Take a centrifuge tube containing 0.34mL whole blood matrix improvement reagent and set aside;
[0048] (2) At 10-30°C, add 10 μL of whole blood to the centrifuge tube containing the whole blood matrix improvement reagent, cover the centrifuge tube tightly, shake and mix quickly, and dilute the whole blood to 35 minutes of the original concentration. One of them is still waiting for inspection;
[0049] (3) On-board detection, the instrument used is the BH5300S atomic absorption spectrometer produced by Beijing Bohui Innovation Optoelectronics Technology Co., Ltd.
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