Matrix modifying reagent for detecting content of lead and cadmium elements in whole blood and application thereof
A technology of matrix improvement and element content, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of high personnel quality requirements, cumbersome operation steps, large blood collection volume, etc., to achieve convenient operation methods, reduce pain, and increase blood collection volume little effect
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Embodiment 1
[0017] Matrix-improving reagents and methods for their preparation and use:
[0018] The matrix improvement reagent formula is as follows: diammonium hydrogen phosphate 5‰, polyethylene glycol octylphenyl ether 2.0‰, concentrated nitric acid 1.0‰, and the rest is ultrapure water.
[0019] Preparation: Weigh 10.00 grams of high-grade pure diammonium hydrogen phosphate, put it in a 2000mL volumetric flask, add 4.0mL polyethylene glycol octylphenyl ether, 2.0mL concentrated nitric acid, then dilute to the mark with ultrapure water, press The specifications are divided into centrifuge tubes to obtain the finished product.
[0020] The method for detecting lead and cadmium element content in whole blood with it comprises the following steps:
[0021] (1) Take a centrifuge tube containing 0.07mL matrix improvement reagent and set aside;
[0022] (2) At 10°C-30°C, add 10 μL of whole blood to the centrifuge tube containing the matrix improvement reagent, cover the centrifuge tube ti...
Embodiment 2
[0025] Matrix-improving reagents and methods for their preparation and use:
[0026] The matrix improvement reagent formula is as follows: diammonium hydrogen phosphate 5.5‰, polyethylene glycol octylphenyl ether 3.0‰, concentrated nitric acid 2.0‰, and the rest is ultrapure water.
[0027] The preparation method is the same as in Example 1.
[0028] The method for detecting lead and cadmium element content in whole blood with it comprises the following steps:
[0029] (1) Take a centrifuge tube containing 0.09mL matrix improvement reagent and set it aside;
[0030] (2) At 10°C-30°C, add 10 μL of whole blood to the centrifuge tube containing the matrix improvement reagent, close the cap of the centrifuge tube, shake and mix quickly to dilute the whole blood to one-tenth of the original concentration , standing still for inspection;
[0031] (3) On the BH2100S / T atomic absorption spectrometer (where S means plastic shell and T means iron shell) produced by Beijing Bohui Inno...
Embodiment 3
[0033] Matrix-improving reagents and methods for their preparation and use:
[0034] The matrix improvement reagent formula is as follows: diammonium hydrogen phosphate 6‰, polyethylene glycol octylphenyl ether 4.0‰, concentrated nitric acid 3.0‰, and the rest is ultrapure water.
[0035] The preparation method is the same as in Example 1.
[0036] The method for detecting lead and cadmium element content in whole blood with it comprises the following steps:
[0037] (1) Take a centrifuge tube containing 0.12mL matrix improvement reagent and set aside;
[0038] (2) At 10°C-30°C, add 10 μL of whole blood to the centrifuge tube containing the matrix improvement reagent, close the cap of the centrifuge tube, shake and mix quickly to dilute the whole blood to 1 / 13 of the original concentration 1. Stand still and wait for inspection;
[0039] (3) Detection by BH2100S / T atomic absorption spectrometer produced by Beijing Bohui Innovation Optoelectronics Technology Co., Ltd.
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