Seed production method of autotetraploid hybrid rice

An autotetraploid, hybrid rice technology, applied in a new field of polyploid heterosis utilization, can solve problems such as retention, and achieve the effects of yield improvement, profound theoretical and practical significance, and huge economic and social benefits

Inactive Publication Date: 2006-11-22
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Looking at the utilization of heterosis, although there have been continuous improvements and innovations in methods and technologies, they still remain at the diploid level and have not been able to break through the limitation of the ploidy level.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: The inventor started to use rice as the material to explore this technology in 1996, using a variety of excellent diploid rice materials such as Minghui 63, RT2248, and 0.1% colchicine at the cellular level. Doubling, has successfully created more than 40 kinds of autotetraploid rice and more than 300 selected lines.

[0034] From 1996 to 2001, the autotetraploid rice materials created by biotechnology were bred in Lingshui County, Hainan Province, and Shuangliu Farm of Chengdu Institute of Biology, Chinese Academy of Sciences. The seed setting rate, fullness and agronomic traits have all been significantly improved. The highest seed setting rate is 90%, and the seed setting rate is over 70%, accounting for one third. Some autotetraploid materials can basically meet the actual production requirements. Needs.

[0035] At the same time, 4 sets of materials for autotetraploid rice male sterile lines and autotetraploid rice male sterile maintainer lines were selected...

Embodiment 2

[0036]Example 2: From 1998 to 2002, in Shuangliu Farm of Chengdu Institute of Biology, Chinese Academy of Sciences, and Wenjiang High-tech Biotechnology Experimental Base of Chengdu Institute of Biology, Chinese Academy of Sciences, a combination of three lines of autotetraploid rice male sterility was carried out. Matching and screening, trial production of nearly 200 combinations, the world's first batch of D46A×D237, D46A×D71 and other super-dominant new autotetraploid hybrid rice combinations, namely super rice. Among them, the yield of the former plot is equivalent to 1015kg per mu, which is 33% higher than the 527 per mu yield of the control D.

[0037] The breeding and seed production experiments carried out by the inventor are similar to those of ordinary diploid rice male sterile three-line breeding and seed production. Currently, it is stepping up field trials to increase its breeding and seed production output.

[0038] Use 8-10ppm ABA, GA3 or 2-5 g / mu of Uniconazole or...

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PUM

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Abstract

The invention belongs to the new technique of polyploid heterosis utilization. Using the autotetraploid material obtained by artificial doubling of excellent diploid rice material, after multiple generations of improvement, the agronomic traits of the improved material meeting the production needs were obtained, and the autotetraploid male sterile three lines were selected. Materials, after further improvement, preparation, screening, and breeding of super-dominant autotetraploid hybrid combinations and new varieties; using autotetraploid male sterile lines and autotetraploid male sterile lines Propagation of breeding lines, using autotetraploid male sterile lines and male sterile restorer lines to produce autotetraploid hybrid rice seeds. The invention integrates the advantages of biotechnology, heterosis utilization and ploidy breeding technology, and the bred autotetraploid hybrid rice has super heterogeneity, strong resistance and adaptability, and has great potential for increasing yield. It opens up a new research field and provides a set of practical and effective technical solutions for realizing the goal of super rice breeding in the new century.

Description

[0001] 1. Field: [0002] The invention belongs to a new technology for utilizing polyploid heterosis. 2. Background technology [0003] Plant polyploidy is widespread in nature. The proportion of polyploidy in gramineous plants is as high as 3 / 4. Polyploidy has the advantages of lushness, largeness, high resistance, strong adaptability, and high effective substance content. It has been a long-term goal pursued by breeders. The discovery of the doubling of colchicine in 1937 opened the door to artificial induction and utilization of polyploidy. Great achievements have been made in the past 60 years. Tetraploid rye, tetraploid sugar beet, tetraploid rubber grass, tetraploid grape, triploid watermelon, hexaploid and octoploid triticale have been used produce. Chinese scholar Bao Wenkui conducted research on rice autotetraploid as early as the early 1950s, and pointed out that rice autotetraploid hybrids may exhibit heterosis. In the 1980s, Chinese scholar Luo Yuewu conducted prelimin...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): A01H1/00A01H1/04
Inventor孔繁伦涂升斌徐琼芳高朝龙
OwnerCHENGDU INST OF BIOLOGY CHINESE ACAD OF S