New use of verbascum glycoside

Verbacoside, a new application technology, applied in the field of polyphenol compound verbascoside

Inactive Publication Date: 2004-03-03
LANZHOU UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, other applications of verbasco

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The nude mice were raised in a laboratory with SPF (Special Isolation of Pathogens) level, and the laboratory was isolated with an air-filtered isolation hood. The feeding, management and use of nude mice were operated in accordance with the "Guidelines for the Management and Use of Experimental Animals" promulgated by the Ministry of Health.

[0017] Table 1 Inhibition of drugs on transplanted human liver cancer tumor mass in nude mice

dosage

(mg / kg)

Tumor volume

(cm3)

tumor weight

(mg)

alpha-fetoprotein

(μg / L)

Control group (n=6)

0×17d

0.62±0.20

613±115

16.0±8.6

Administration group (n=6)

200×17d

0.16±0.15 **

198±184 **

4.9±4.8 **

[0018] * p** p<0.01, n is the number of experimental mice

[0019] The results show that the medicament of the present invention can obviously inhibit the growth of the tumor mass, and the volume and weight...

Embodiment 2

[0021] Human gastric adenocarcinoma cells (MGc80-3) were first incubated (that is, cultured in a cell incubator with a temperature of 37°C and 5% carbon dioxide) in RPMI-1640 culture medium with a concentration of 20 μmol / L verbascoside. The same amount of human gastric adenocarcinoma cells were cultured in RPMI-1640 culture medium containing DMSO without drug as the control group. Cells were collected after 7 days of incubation, washed 3 times routinely, and made into 5×10 7 / mL of cell solution. Take 8 healthy nude mice, inject 5 × 10 6 cells, and inject the same amount of cells from the control group into the other side of the buttock of each mouse as a control. After four weeks of normal feeding, the control side of the 8 nude mice all grew heavier tumors, with an average weight of about 1g, while only one mouse on the drug-treated side grew a tumor weighing about 0.5g. This experiment shows that the present invention can make human gastric cancer cells lose malignancy ...

Embodiment 3

[0023] Human gastric adenocarcinoma cells (MGc80-3) and human gastric cancer cells (MKN45) were used as experimental materials in this example.

[0024] will be 5×10 4 / mL human gastric adenocarcinoma cells were put into RPMI-1640 culture medium and cultured at 37°C and 5% carbon dioxide for 24 hours. After the culture medium was discarded, these gastric adenocarcinoma cells were divided into two parts, one was cultured in RPMI-1640 culture medium containing 20 μmol / L verbascoside, and the other was cultured in RPMI-1640 culture medium containing no verbascoside but containing 200 mmol / L LDMSO Cultured in RPMI-1640 medium as a control. After incubation for 7 days, the cells were harvested, washed three times with D-Hank's solution, and made into 1×10 6 cells / mL of cell fluid. Cell surface charge and cell number doubling time were measured. Another part of the cells was cultured on the coverslip, fixed with 0.1 mol / L phosphate buffer containing 2.5% valerylaldehyde, pH 7.4 ...

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PUM

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Abstract

An application of verbascum glycoside in preparing the medicines for treating cancers by oral application or injection is disclosed.

Description

technical field [0001] The invention relates to a use of polyphenol compound verbascoside. Background technique [0002] Verbacoside is a polyphenolic compound extracted from Scrophulariaceae. Its molecular formula is C 29 o 15 h 36 , the molecular weight is 624, and the physical constants of the structure are: [0003] Specific rotation: [α] D 20 , -68 (C=0.4, MeOH) [0004] Ultraviolet: UVλ max MeOH (nm): 208 (lgε4.30), 217 (lgε4.45) [0005] 292 (lgε4.10), 329 (lgε4.25) [0006] Infrared: IR v max KBr (cm -1 ): 3400 (OH), 2932 (C-H), 1700 (conjugated ester group), 1631 (C=C), 1604, 1521 (aromatic ring). [0007] Hydrogen spectrum: 1 HNMR (400MHz, DMSO-d 6 , TMS) δppm: 0.97 (d, J=6.0Hz, 3H, methyl group of rhamnose), 2.70 (t, J=7.0Hz, 2H, Ar-CH 2 -CH 2 ), 4.36 (d, J=7.2Hz, 1H, H-1 of glucose), 4.72 (t, J=9.6Hz, 1H, H-4 of glucose), 5.03 (d, J=1.1Hz, 1H, mouse H-1 of Li sugar, 6.21 (d, J=15.8Hz, 1H, Ar-CH=CH), 6.49-7.01 (6H, aromatic ring H), 7.47 (d, J=1...

Claims

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Application Information

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IPC IPC(8): A61K31/7034A61P35/00
Inventor 贾忠建郑荣梁
Owner LANZHOU UNIVERSITY
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