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Targetted polypeptide for specificity of liver cancer blood vessel

A technology for tumor blood vessels and targeting peptides, which is applied in the direction of anti-tumor drugs, peptides, peptide/protein components, etc.

Inactive Publication Date: 2004-12-08
ZHONGSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are no reports of targeting molecules that can specifically target tumor blood vessels in liver cancer at home and abroad

Method used

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  • Targetted polypeptide for specificity of liver cancer blood vessel

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The present invention screens and obtains LCI-X through the following methods and steps 7 peptide.

[0018] 1. Establishment of human liver cancer orthotopic nude mouse model:

[0019] Using highly metastatic human liver cancer cell line MHCC97-H (World J Gastroenterol2001, 7: 630-636) 1×10 7 Tumors were formed by subcutaneous injection in male BALB / c nu / nu nude mice (purchased from the Institute of Materia Medica, Chinese Academy of Sciences). After the subcutaneous tumors grew to 1-1.5 cm, the subcutaneous tumors were removed, cut into 1mm×1mm×1mm, and planted in nude mice in situ Liver, when the tumor grows to about 1cm;

[0020] 2. Random phage library screening in vivo:

[0021] Mice were injected into the tail vein of f1 phage 10 12 Pfu, injected into phage peptide library 5 minutes later, thoracotomy after 5 minutes, perfused with 20ml PBS containing heparin anticoagulant blood in vivo heart, portal vein and subhepatic inferior vena cava cannula supplemented ...

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Abstract

A liver cancer blood vessel specific target polypeptide LCI-X7 able to specific binding with liver cancer blood vessel is extracted from the naked mouse model of human liver cancer by the showing technique of phage random peptide library in body and microscopic laser cutting technique. It provides important theory and practical basis for the diagnosis to liver cancer transfer recurrence and target therapy.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to protein polypeptides, in particular to a polypeptide that can specifically bind to blood vessels of liver cancer tumors. Background technique [0002] my country is a high-incidence area of ​​liver cancer in the world. According to statistics, about 200,000 people die of liver cancer every year, accounting for about half of the global liver cancer deaths. Modern medicine has conducted scientific research on liver cancer for more than a hundred years. Although many achievements have been made, the overall 5-year survival rate of liver cancer patients is still only about 3%. The biggest obstacle to improving the prognosis of liver cancer is its extremely high invasion and metastasis. Even with radical surgical resection, 50% of patients will develop metastasis or recurrence within 5 years. Therefore, in-depth study of the internal mechanism of liver cancer metastasis and recurrence and the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/08A61P1/16A61P35/00C07K7/06
Inventor 徐泱孙惠川汤钊猷钦伦秀刘银坤
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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