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Gene chip used for respiratory tract virus monitoring

A technology of gene chip and respiratory tract, applied in the field of gene chip, can solve the problems of detection of a single, time-consuming method of virus culture, unsuitable for on-site detection, etc.

Inactive Publication Date: 2005-05-25
北京金迪克生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned detection methods still have great limitations. First, only one single virus can be detected at a time; second, the specificity and sensitivity of the detection method still have certain limitations; On-site testing

Method used

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  • Gene chip used for respiratory tract virus monitoring

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Experimental program
Comparison scheme
Effect test

Embodiment approach 2

[0090] Embodiment 2 Labeling Fluorescent Dyes

[0091] 1. The first step PCR: add 5ul template nucleic acid, 2ul10*PCR buffer, 0.6ul dNTP (10mM), primer (100pmol / μl) 1ul, dH into the reaction tube 2 O10.4ul, Tag (5U / μl) 1μl. Mix and centrifuge. The procedure is as follows:

[0092] 94℃ 5min

[0093] 94℃ 1min

[0094] 30℃ 5min

[0095] 0.1℃ / s rises to 72℃

[0096] 72℃ 3min

[0097] 5 cycles

[0098] 2. The second step PCR

[0099] Template 5μl

[0100] 10*PCR buffer 5μl

[0101] Primer-2(100pmol / μl) 2μl

[0102] dNTP (10mM) 2 2μl

[0103] dH 2 O 34μl

[0104] Tag(5U / μl) 2μl

[0105] Mixing; centrifuging

[0106] The procedure is as follows:

[0107] 94℃ 5min

[0108] 94℃ 1min

[0109] 40℃ 5min

[0110] ...

Embodiment approach 5

[0137] Embodiment 5 Scanning and analyzing results The chip was scanned with GenePix 4100A scanner. Use Genepix Pro 5.0 to extract data and analyze the results.

Embodiment approach 6

[0138] Implementation Plan 6 Monitoring Results of Clinical Specimens

[0139] The clinical specimens of 30 SARS patients collected in Beijing Xuanwu Hospital and Beijing You'an Hospital were tested. The results of the patient tests were all positive (see the attached respiratory virus monitoring gene chip), indicating that the monitoring results are satisfactory.

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Abstract

A biochip for detecting the respirovirus features that the conservative probe is designed by biologic calculation and analysis, the gene detection chip is prepared, and a technique platform able to sensitively, specifically and quickly detect various respiroviruses at the same time is created by crossing and scan analyzing.

Description

Invention field [0001] The invention relates to a gene chip for respiratory virus monitoring. It is used for the monitoring of respiratory tract viruses in patient specimens and ambient air, and the platform technology established by it can also be used in the field of anti-bioterrorism involving national security. Background of the invention [0002] Gene chip (gene chip) is also called DNA chip, DNA microarray (DNA microarray), oligonucleotide array (oligonucleotide array), refers to the use of in situ synthesis (in situ synthesis) or micro-printing means, tens of thousands of The DNA probes of the meter are solidified on the surface of the support to produce a two-dimensional DNA probe array, which is then hybridized with the labeled sample. The hybridization signal is detected to achieve rapid, parallel, and efficient detection or medical diagnosis of biological samples. The silicon chip is used as a solid support, and the preparation technology of the computer chip is used i...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 孙梅生舒跃龙王征侯云德
Owner 北京金迪克生物技术有限责任公司
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