Hematopoietic stem cell culturing apparatus and method

Abstract

The present invention relates to apparatus and method of culturing mammal cell, and is especially culturing apparatus and method for selective amplification of human stem cell, including hemopoietic stem cell. The apparatus and method of the present invention may be used widely in biological treatment of tumor and transplantation of hemopoietic stem cell.

Description

technical field [0001] The invention relates to a device and method for growing mammalian cells in culture, in particular to a device and method for selectively expanding human stem cells and hematopoietic stem cells in vitro. The device and method can be widely used in tumor biotherapy and hematopoietic stem cell transplantation. Can be widely used in various clinical biological cell therapy. technical background [0002] The possibility of using cells for a wide range of different therapeutic purposes has always been a focus of interest in the biomedical community. The human hematopoietic system produces a variety of cells to protect the body from pathogens, toxic substances, and other harmful agents. The hematopoietic system originates from simple stem cells, which are induced to produce the hematopoietic system. The principle and process of how human stem cells are induced to differentiate into the hematopoietic system have not been fully understood. Once the stem cell...

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Problems solved by technology

[0021] In the past bioreactor design, although the cell harvesting procedure was considered as an important aspect, the proposed method is still a wide-opening open-type structure, the culture process must be stopped when the cells are harvested, and the culture chamber must be exposed. In the atmosphere, this structure is prone to contamination of the culture chamber

[0022] In cell culture, the bioreactor needs to maintain an internal equilibrium state, especially in the complex original cell culture process, human stem cells or human hematopoietic stem cells are very sensitive to nutrient perfusion rate (ie gas perfusion rate / nutrient solution perfusion rate / growth factor perfusion rate), is also very sensitive to the supply and removal of the above substances, and is also sensitive to the chemical environment of the culture. In the current bioreactor, there is no design that can meet the above requirements, so as to maintain the balance and stability of the culture internal environment. , especially during primary cell culture

[0023] The current design does not address a method for harvesting cells without interrupting the culture, nor does a design maintain a balanced culture environment while providing the dual advantages of stable gas and liquid perfusion rates Not found

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