45 results about "Mammotroph" patented technology

The invention discloses a method for targetedly integrating an exogenous gene into a target gene of a mammal, which is implemented by targetedly integrating an exogenous gene into a target gene by utilizing a CRISPR/Cas9 system, and especially targetedly integrating a Cre gene to a first exon of a mouse Enpp1 gene. The technique has the advantages of high integration efficiency (20%) and simple operation step, only needs to construct one exogenous gene vector, and can not be influenced by the position effect.

The objective of the invention is to provide a photo-aging prophylactic agent for skin with novel ingredients and foods using the photo-aging prophylactic agent for skin, medicaments for mammals including human beings and a skin external agent, as well as feed for mammals. The inventive photo-aging prophylactic agent for skin is characterized by taking extracts of Orobanchaceae plants as effective ingredients. The photo-aging prophylactic agent for skin preferably comprises phenylethanoid glycosides compounds as effective ingredients. Moreover, the photo-aging prophylactic agent for skin preferably comprises at least one of echinacoside and acteoside as the phenylethanoid glycosides compounds. On top of that, the photo-aging prophylactic agent for skin preferably comprises both echinacoside and acteoside as the phenylethanoid glycosides compounds. In addition, the photo-aging prophylactic agent for skin can be employed as medicaments for mammals including human beings, skin external agents, consumptive foods and feed for mammals.

The present invention relates to antibiotic dressing for treating injury of mammal, especially human body, and is especially a kind anti-coagulation nano silver antibiotic dressing prepared via anti-coagulation technology. The antibiotic dressing includes a kind of fabric and composite nano silver particles deposited onto the fabric, and the composite nano silver particles in the size of 1-100 nm are attached firmly between the fiber elements and on the fiber. The composite particles have core of metal silver and surface layer of silver oxide, which accounts for 10-80 wt% of the whole composite particles, and the silver content of the dressing is less than 50 microgram each squared millimeter.

The invention discloses an electroporation method for transfection of mammal embryo with siRNA (small interfering Ribose Nucleic Acid). The electroporation method comprises the following steps: (1) weakening an embryo zona pellucid; (2) preparing electroporation solution containing siRNA; and (3) conveying the embryo into the prepared electroporation solution containing siRNA, standing at the room temperature, and then conveying the embryo and the electroporation solution into an electric turn trough for electroporation. The electroporation method comprises the steps of weakening the embryo zona pellucid and complexing the siRNA, and then placing the embryo under the action of a pulsed electric field to increase the permeability and the membrane conductance of the embryo instantaneously, and thus the siRNA which is difficult to penetrate through a cell membrane under the normal physiological condition enters the cell. Statistic observation and analysis prove that the stable positive transfection efficiency of survival embryos exceeds 95%, and the practical effective utilization rate of the collected total embryo exceeds 70%. The electroporation method is a safe, simple and efficient transfection technology of mammal embryo with siRNA.

The invention discloses a lyrica preparation method. Lyrica is an analogue of a mammal neurotransmitter of Lambda-propalanine, is clinically used for curing diabetic peripheral neuralgia and post-herpetic neuralgia and adjunctively treating partial adult seizure. The Lyrica is synthesized through using S-(+)-leucine as a raw material by a chemical method without decomposition, and has the advantages of easily obtained raw materials, less reaction steps, mild reaction conditions, high reaction yield, and the like.

The invention relates to a composition and kit for quickly extracting circulating un-related karyocyte from peripheral blood of an animal disease model, as well as application thereof. The composition which is used for quickly acquiring circulating un-related karyocyte from mammal blood of an animal disease model (including a tumor animal model, a cardiovascular disease animal model and the like consists of buffer fluid, magnetic or non-magnetic immunomicrospheres coated by anti-related karyocyte surface marker antibody, and cell separation medium with certain density. Compared with commercial centrifugal medium which is universally applied currently on the market, the centrifuge time can be greatly shortened by applying the novel composition, and the cell recovery rate is stably increased to over 95 percent from the previous 60+/-20 percent. The erythrocyte is not required to be cracked, molten and broken by means of the method, so that the un-related circulating rare cells can be enriched and extracted from the peripheral blood in the animal disease model effectively and quickly.

The invention provides a drug of bone-melon extract for injection, mammalian bone peptides and melon seed peptides are extracted, the obtained extracts are mixed according a certain proportion, thus preparing the drug for injection. The invention adopts a special extraction technology to increase the active ingredients, the extraction efficiency is high, and the substances with high molecular weight are reduced to the minimum. The drug of the invention has the effects of regulating bone metabolism, stimulating proliferation of osteoblasts, promoting formation of new bones, regulating calcium and phosphorus metabolism, increasing bone calcium deposition and preventing osteoporosis, thus being used for prevention and treatment for rheumatism, rheumatoid arthritis, osteoarthritis, lumbar and leg pain, fracture trauma repair and other diseases.

The invention relates to a magnetic resonance imaging contrast agent, which uses paramagnetism manganese heteropoly complex, wherein the inventive contrast agent is the high-stability chelate formed by transition paramagnetism manganese and heteropoly acid or heteropoly acid salt with Keggin or Dawson structure, which has target property and better signal strengthen effect on the liver, kidney, etc. And the inventive chelate can be used in X-ray CT or ultrasonic image contrast.

The invention relates to a novel application of aromatic nitro compound in the pharmaceutical field, in particular to the application of aromatic nitro compound in treating viral hepatitis. The aromatic nitro compound can be used alone or can be combined with other commonly used anti- viral hepatitis drugs to effectively restrain the replication and secretion of hepatitis virus. The aromatic nitro compound can remarkably restrain the replication and secretion of hepatitis virus in the bodies of mammals. The aromatic nitro compound is especially suitable for treating the diseases caused by hepatitis B virus and hepatitis C. The invention provides the concrete method for treating the patients with hepatitis B and hepatitis C.

The present invention is dibutyrul adenosine cyclophosphate calcium and its hydrate in the molecular formula of C18H23N5O8P.1/2Ca.nH2O, where n=0-2 5 of different characters. Dibutyrul adenosine cyclophosphate calcium is slightly soluble in water at room temperature and insoluble in ethanol and has high storage stability. Dibutyrul adenosine cyclophosphate calcium and its hydrate are used as protein kinase activator for treating cardiac and cerebral vascular diseases mainly as well as in regulating growth of mammals.

The invention discloses a new utility of hepatitis B surface antigen-antibody compound in the hepatitis B vaccine non-respond or low-respond prevention agent, which is characterized by the following: purifying recombined HBsAG or inactivated serum HBsAG in the mammal cell, adopting hepatitis B with antibody serum or vaccine globulin as compound, improving anti-HBs ability for non-respond or low-respond mouse.

The invention relates to a rhizoma dryopteris crassirhizomae and fructus crataegi composition, a preparation method and an application of the composition. The effective components of the composition provided by the invention are prepared from the following raw materials by weight: 10-90 parts of rhizoma dryopteris crassirhizomae and 10-90 parts of fructus crataegi. The rhizoma dryopteris crassirhizomae and fructus crataegi composition provided by the invention is good in effect of preventing and treating poulty viral diseases, in particular to the poultry diseases caused by bursal disease viruses, the poultry kidney diseases caused by renal type infectious bronchitis viruses, the influenza virus A diseases of mammals caused by avian influenza viruses and the poultry avian influenza virus diseases; and the rhizoma dryopteris crassirhizomae and fructus crataegi composition provided by the invention has the characteristics of being more liable to obtain the raw materials, good in curative effect, free of residues, green and environment-friendly and the like, so that the composition can replace the chemical antiviral drugs.

The invention relates to the use of effective amount of Pelargonium graveolens essential oil or extract or constituents thereof selected from a group consisting of: (S)(-)citroneIlol, linalool, menthone and isomenthone or any combination thereof, in the preparation of a medicament for treating a mammal suffering from or susceptible to a neurodegenerative condition which can be improved or prevented by inhibition of acetylcholinesterase (ACliE).

Provided is a composition comprising an extract from oil palm leaves characterized in that the extract comprises (-)-catechin gallate, ferulic acid, and phenolic acids such as gallic acid and protocatechuic acid and a method for producing said extract comprising the steps of (a) extracting the dried or fresh oil palm leaves with a solvent, (b) filtering the extract obtained in (a), (c) contacting the filtered extract from (b) with an chromatographic medium which selectively adsorbs a fraction containing (-)-catechin gallate, ferulic acid, and phenolic acids, (d)eluting the said fraction from the chromatographic medium using a solvent, (e) drying the eluted fraction obtained in step (d). Also claimed is the use of the composition for reducing or preventing oxidative stress in mammals and poultry.

The invention relates to a cognitive map construction method based on space and motion joint coding, and belongs to the technical field of robot navigation. A sniffing layer-hippocampus neural circuitin an internal positioning system of the brain of a mammal is considered; a continuous attractor neural network model-spatial memory network is formed by utilizing spatial navigation coding characteristics of position cells, head orientation cells, grid cells and velocity cells, space and motion characteristics of animals are coded at the same time, and path integration is carried out on angularvelocity and linear velocity. In the network, the asymmetric connection weights among the neurons generate system inherent activity peaks capable of spontaneously moving, once speed input exists, theattractor network can steadily integrate the linear speed and the angular speed, and stable robot head direction and position coding is formed. And local view cells are formed through single-camera visual input, so that the accumulative error of path integration can be corrected. According to the invention, a consistent half-meter topological map can be stably constructed by using a single camera.

The invention discloses a maternal inheritance of acquired character -phenotype of acquired character test animal model test method and relates to the fields of life science and medicines. The invention provides a test animal model method capable of breeding-cultivating higher mammals with spontaneous diseases applied to simulating human diseases. The descendant test animals with metabolic syndrome-hyperglycemia and diabetes mellitus are obtained by feeding gestational mother animals with high-energy feed and are applied to contrast experiment detection/analysis to obtain a biological energy metabolism regulation and control pathogenesis mechanism and a treatment mechanism of human metabolic syndrome-hyperglycemia, diabetes mellitus, obesity and the like, develop new generation treatment medicines and guide clinical intervention treatment. Existence of 'inheritance of acquired character' is confirmed, so that the conventional modern biological foundation/applied science theory method is overturned fundamentally and completely.

The invention discloses an in-vitro culture and passage method of buffalo spermatogonial stem cell-like cells. The in-vitro culture and passage method comprises the following steps: putting primary buffalo spermatogonial stem cell-like cells into a culture dish containing buffalo supporting cells; culturing for 60 to 120 h to finish in-vitro culture; adding a PBS (Phosphate Buffer Solution) into the buffalo spermatogonial stem cell-like cells obtained by the in-vitro culture; collecting superficial cells; adding a pancreatin cell digestive juice into the superficial cells and digesting for 10to 60 s; then adding an IMDM (Iscove's Modified Dulbecco's Medium) culture solution to stop enzymic digestion reaction; collecting superficial cells and transferring the superficial cells which are collected at the second time into a culture dish paved with gelatin; culturing for 1.5 to 4 h; taking supernatant cells and transferring into a feeder layer containing the buffalo supporting cells to finish passage culture. The in-vitro culture and passage method provided by the invention is simple and convenient to operate, safe and reliable and high in repeatability, and has great significance ontreatment of infertility of mammals and protection of endangered species resources.

The invention discloses an integrated hypoxia experiment simulation device and a method of mammals. The integrated hypoxia experiment simulation device comprises a processor module; the processor module is connected with a display module, a setting module, a sensor module, a power module and a control module; the control module is connected with a vacuum pump. The integrated hypoxia experiment simulation device provided by the invention has the characteristics of being compact in structure and convenient to use; besides; the device can automatically control vacuum pressure, reduce the operating difficulty of students making experiments, and enable the students to mainly focus on the observation of the hypoxia phenomenon, so that the experiment efficiency is greatly enhanced, and the experiment effect is improved; moreover, the experimental device is stable in experiment effect, high in repeatability and good in effect of experiment teaching.

The invention relates to an application of benzoquinone compounds in improving antioxidase expression of mammals to suppress pathology phenomens related to inflammations, and a medical composition and a supplement which are prepared by the benzoquinone compounds. The benzoquinone compounds comprise a structure shown as a compound (I). In the compound (I), R1 represents a hydrogen group or [(C4H4)CH3]n; n is an integer ranging from 1 to 5; and R2 represents a hydrogen group or a methoxy group.

The invention provides donkey-hide gelatin containing black-bone sheep and a preparation method of the donkey-hide gelatin containing the black-bone sheep. The black-bone sheep is added during preparation of the donkey-hide gelatin, and raw materials include black-bone sheepskin and bones, donkey hide, Radix Angelicae Sinensis, Rhizoma Ligustici Wallichii, Radix Aconiti Carmichaeli and Rhizoma Zingiberis. By combination of high-quality black-bone sheep gelatin and traditional donkey-hide gelatin, selection of the raw materials is in coordination with black donkeys which are superior raw materials of the donkey-hide gelatin, and the black-bone sheep which is rich in melanin has dual healthcare functions of Yin nourishing and Yang tonifying, is the only known mammal high in in-vivo melanin content and has extremely high medicinal and healthcare values. The black-bone sheep gelatin and the donkey-hide gelatin are compatible and complementary, quality and nutrition of the traditional donkey-hide gelatin are improved, deep development of related black-bone sheep products is greatly promoted, black-bone sheep breeding and industrial development can be promoted, and local economy is promoted substantially.

The invention relates to a brainlike robot navigation method based on Bayes multi-mode perception fusion. The brainlike robot navigation method based on Bayes multi-mode perception fusion comprises following steps: integration cells are subjected to vestibular information fusion; integration cell spike rate is changed based on obtained vestibular information; calibration cells are subjected to visual sense information fusion; grid cell network and head direction cell network are subjected to energy injection, and integration cell and calibration cell spike rates are changed; comprehensive inhibition is carried out; and the phases of the spike rates of the grid cell network and the head direction cell network are estimated so as to obtain the current robot practical position and head direction; and a topological map is constructed. A model disclosed in the invention can be used for multi-mode perception fusion, realizing stable coding on robot space environment and robot self states; the head direction neuromechanisms of the model and mammals are the same, the results are similar to mononeuron recorded experiment results acquired in neurobiology, and extremely high biological fidelity is achieved.