Composition and kit for quickly extracting circulating un-related karyocyte from peripheral blood of animal disease model, as well as application thereof

Abstract

The invention relates to a composition and kit for quickly extracting circulating un-related karyocyte from peripheral blood of an animal disease model, as well as application thereof. The composition which is used for quickly acquiring circulating un-related karyocyte from mammal blood of an animal disease model (including a tumor animal model, a cardiovascular disease animal model and the like consists of buffer fluid, magnetic or non-magnetic immunomicrospheres coated by anti-related karyocyte surface marker antibody, and cell separation medium with certain density. Compared with commercial centrifugal medium which is universally applied currently on the market, the centrifuge time can be greatly shortened by applying the novel composition, and the cell recovery rate is stably increased to over 95 percent from the previous 60+ / -20 percent. The erythrocyte is not required to be cracked, molten and broken by means of the method, so that the un-related circulating rare cells can be enriched and extracted from the peripheral blood in the animal disease model effectively and quickly.

Description

technical field [0001] The present invention mainly relates to a composition and a kit for rapidly extracting circulating non-blood nucleated cells from the peripheral blood of animal models of diseases, and the composition or kit is used in the research and development of new drugs and the search for new biomarkers such as Application of tumor markers and other biomedical experiments based on animal models of diseases, manual or automatic detection equipment operations. Background technique [0002] The great clinical significance of extracting and detecting circulating non-blood-related nucleated cells (such as vascular endothelial cells, tumor epithelial cells) in peripheral blood has long been confirmed by people. The current commonly used method for extracting circulating non-blood-related nucleated cells is to use antibodies to combine with special markers on the surface of these cells to achieve the purpose of direct capture. However, this direct capture technique ha...

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Problems solved by technology

Although some single methods involved in this method have been reported, such as the immunomagnetic particle method to remove white blood cells, the blood glycophorin-A (glycophorinA) cross-linking method to remove red blood cells, and the immunomagnetic particles and Preliminary attempts to combine density centrifugation, etc., but these methods are time-consuming, have low leukocyte removal rate, poor target cell recovery rate, and require the use of special equipment, which brings inconvenience to application