Biochemical reaction apparatus, biochemical reaction substrate, process for producing hybridization substrate and hybridization method

Abstract

A bioassay substrate (1) is flat and has a disc-shaped main side like an optical disc such as CD. The substrate (1) is rotatable about a central hole (2) formed therein. The substrate (1) has formed on the surface (1a) thereof a plurality of wells (8) where a probe-use DNA (detection-use nucleotide chain) and sample-use DNA (target nucleotide chain) react with each other for hybridization. The substrate (1) has a transparent electrode layer (4) formed as an underlying layer of the well (8). For hybridization, an external electrode (18) is placed in a position near the transparent electrode layer (4) from above the top surface (1a) of the substrate (1) to apply an AC power to between the transparent electrode layer (4) and external electrode (18) in order to apply an AC electric field perpendicularly to the substrate (1).

Description

technical field [0001] The present invention relates to a biochemical reaction device that provides a biochemical reaction by using a substrate, a substrate for a biochemical reaction (hereinafter also referred to as a bioassay substrate), such as a DNA chip, etc., a method of hybridizing a nucleotide chain, and a detection method wherein A method of manufacturing a substrate for hybridization in which a probe is immobilized with a nucleotide chain. [0002] This application claims priority from Japanese Patent Application No. 2003-193064 filed in Japan Patent Office on Jul. 7, 2003, the entire contents of which are hereby incorporated by reference. Background technique [0003] In recent years, substrates for biochemical reactions called "DNA chips" or "DNA microarrays" (hereinafter referred to as DNA chips") have been used to analyze gene mutations, SNPs (single nucleotide polymorphisms), phenomenon, single nucleotide polymorphisms) and the frequency of gene expression (g...

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Problems solved by technology

[0006] However, since the single-stranded DNA does not form the usual strands in solution, but forms random coils, thereby sterically hindering the binding of sample DNA and probe DNA, thereby making it difficult to quickly hybridize DNA

Even under the action of the electric field, the drifting sample DNA moves towards the probe DNA, the steric hindrance still exists, and it is difficult to perform faster hybridization

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