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Female-specific AFLP fragment of Cynoglossus semilaevis Gunther and PCR method for identification of genetic sex

A semi-smooth tongue sole, female technology, applied in the field of aquatic organisms, can solve problems such as unreported

Inactive Publication Date: 2006-12-20
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no reports at home and abroad on the PCR method for female-specific DNA markers and genetic sex identification of Soleidae fishes.

Method used

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  • Female-specific AFLP fragment of Cynoglossus semilaevis Gunther and PCR method for identification of genetic sex
  • Female-specific AFLP fragment of Cynoglossus semilaevis Gunther and PCR method for identification of genetic sex

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Embodiment Construction

[0020] Using molecular cloning technology to clone sex-specific AFLP fragments and establish a conventional PCR analysis technology for genetic sex identification of tongue sole, which is helpful for breeding all-female seedlings of tongue-sole half-smooth sole, raising all-female seedlings, increasing the breeding yield of tongue-sole half-smooth sole, and improving the economy of breeding It has important practical significance and huge economic benefits to really develop tongue sole into an excellent cultured species accepted by the majority of farmers, promote the development of tongue sole aquaculture industry and the replacement of marine fish culture species.

[0021] The technical content of the present invention will be described in detail below in conjunction with the accompanying drawings through the embodiment of 'cloning of the female-specific AFLP fragment of half-smooth tongue sole and the establishment of a PCR method for genetic sex identification':

[0022] 1....

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PUM

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Abstract

The invention discloses a semi-sliding-tongue halibut female astopic AFLP segment and PCR authenticating method of genetic sex, which comprises the following steps: recycling female astopic AFLP segment; cloning AFLP segment; analyzing sequence; authenticating semi-sliding-tongue halibut through PCR method; establishing to separate and clone semi-sliding-tongue halibut female astopic AFLP segment; obtaining female astopic DNA segment; designing astopic primer; transmitting AFLP mark into SCAR mark; creating PCR technology for genetic sex authentication of semi-sliding-tongue halibut.

Description

Technical field: [0001] The invention belongs to fish genetic sex identification and sex control technology in the technical field of aquatic organisms, and is a molecular biological method for fish genetic sex identification that can be applied in fish parthenogenesis. Background technique: [0002] my country is rich in fish resources, many of which have gender differences in growth rate. For example, male tilapias grow about 40% faster than females, while seawater fish such as flounder and half-smooth tongue sole, females grow faster than males. Individuals grow 40%-100% faster. Therefore, research on genetic sex detection and sex control of these fishes has important scientific significance, great application potential and promotion prospects. [0003] Cynoglossus semiliaevis (Cynoglossus semiliaevis) is a rare and valuable seawater fish endemic to my country. It belongs to warm-water demersal fish in coastal waters. It is distributed in coastal areas of my country, mostl...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 陈松林李静田永胜沙珍霞王清印庄志猛徐建勇
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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