Method for Characterizing a Sample by Data Analysis
a data analysis and sample technology, applied in the field of sample characterization, can solve problems such as corruption of measuring results, and achieve the effect of reliable and easily realisable methods
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first exemplary embodiment
[0111]200 μl of a buffer solution were added to 500 μl human urine. This buffer solution consists of a sodium phosphate buffer having a concentration of 800 mmol per litre and a pH value of 7.4, 300 μmol per litre sodium trimethylsilyl propionate (TSP) and 15% D2O, wherein the nominal pH value was measured prior to D2O addition. Urine and buffer were mixed in an Eppendorf tube and centrifuged for 15 minutes at 4° C. and 17.900 rpm. 500 μl of the supernatant were transferred into a 5-mm NMR sample tube. Subsequently, an NMR measurement took place.
[0112]An NMR spectrometer of the type Advance II+600 having a TXI sample head was used for NMR measurement. The height of the sample tube in the spinner was 20 mm and the adjusted actual temperature 298 K. The maximum storage time of the sample at room / measuring temperature was four hours. The measurement was started when a sufficient temperature equilibration was present; this was the case if the temperature fluctuation of the sample was wi...
second exemplary embodiment
[0135]A “Nespresso” coffee capsule containing ca. 5 g coffee powder was inserted into a “Nespresso” coffee machine. The coffee powder was extracted by ca. 120 ml hot water according to the standard extraction proceedings performed by the coffee machine. Ca. 118 ml liquid coffee extract were obtained. 50 μl sodium phosphate buffer and 25 μl NMR standard (consisting of 2 mmol / l trimethylsilyl propionate [TSP] in D2O) were added to 425 μl of the coffee extract. This mixture was then transferred into a 5-mm NMR sample tube for the subsequent NMR measurement.
[0136]An NMR spectrometer of the type Advance II+600 having a TXI sample head was used for NMR measurement. The height of the sample tube in the spinner was 20 mm and the adjusted actual temperature was 298 K. The maximum storage time of the sample at room / measuring temperature was four hours. The measurement was started when a sufficient temperature equilibration was present; this was the case if the temperature fluctuation of the s...
third exemplary embodiment
Varieties
[0162]Mustard seeds contain hundreds of compounds. The ratio of these compounds is determined by growth conditions (environmental influence) and the belonging to a certain variety (genetic influence). A small part of a very complex metabolism network is suited to determine the belonging of a mustard seed to a certain variety.
[0163]The inventors were able to show that 50 features of the complex metabolism network are suited for an according classification (i.e. a clarification of the respective variety).
[0164]Mustard seeds to be analyzed were chosen by hand to exclude the possibility of contamination of the sample material by small stones, stipes, grains of foreign seed or seeds which are atypically coloured, broken up, germinating or foul.
[0165]The chosen seeds (ca. 2 g) were ground in a mixer mill for 5 minutes at 30 Hz. The ground stock was transferred after grinding into a sealable polypropylene container and—if not immediately used for measurement—stored at 4° C. for no...
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Abstract
Description
Claims
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