Nematode-trapping fungus powder preparation and application thereof

Abstract

The invention belongs to the technical field of microbiology and particularly relates to a nematode-trapping fungus powder preparation and application thereof. Nematode-trapping funguses are funguses for nematode trapping by a predator formed by vegetative hyphae specialization, are natural enemies of nematodes and have the special ability to capture and kill the nematodes. Therefore, the great potential means of prevention and treatment is to utilize the nematode-trapping funguses to perform biological prevention and treatment on larva at the free-living stages of parasitic nematodes in domestic animals so as to replace a chemical insect repellent. The preparation involved in the invention is simple in preparation process, low in production cost and more conducive to mass production, and matrix corns, wheat and the like produced by chlamydospore are animal edible feed, so that the waste is avoided; the powder preparation is added into the feed or directly eaten by the animals, and is good in palatability; meanwhile, the powder preparation has the characteristics of being easy to package, save and transport and convenient to apply to the production.

Description

technical field

[0001] The invention belongs to the technical field of microbiology, and in particular relates to a nematode-predating fungus powder preparation and its application. Background technique

[0002] Gastrointestinal nematode disease is the most common parasitic helminth disease in livestock, which is distributed worldwide and causes serious economic losses to the animal husbandry industry. For a long time, it has mainly relied on chemical anthelmintics to control gastrointestinal nematodes in livestock. With the continuous use of anthelmintics, the resistance of nematodes to anthelmintics has continued to increase, and it has caused environmental pollution and residues of chemical drugs in livestock products. At present, the resistance genes of animal parasitic nematodes to anthelmintics are widely spread in the breeding industry all over the world, which has caused a huge threat to animal husbandry production. People are actively looking for natural plant reso...

Examples

Embodiment 1

[0017] (1) Preparation of nematode-predating fungus powder preparation

[0018] (1) Isolation, purification, isolation and identification of nematode-predating fungal isolates SDH035 and F088 From September 2013 to August 2014, samples from sheep manure piles in Wuzhong area of ​​Ningxia and sheep identification samples in Yichun City, Heilongjiang Province, Separation using the improved plate spreading method. During separation, add the third stage larvae of sheep nematodes as bait to the plate and incubate at 25°C. Check under an inverted microscope once a day or every other day for 3 weeks to pick out a single predatory structure or prey Nematodes, transferred to 0.2% bran agar and cultured at 25°C for 3 to 14 days. After the conidia grew, pick a single conidia and put it on another 0.2% bran agar plate until a pure culture was obtained. . The morphology and data measurement of the conidia of the strain were obtained by the slide insertion method, and the DNA of the strain...

Embodiment 2

[0038] (1) Preparation of nematode-predating fungus powder preparation

[0039] (1) Isolation, purification, isolation and identification of nematode-predating fungal isolates SDH035 and F088 From September 2013 to August 2014, samples from sheep manure piles in Wuzhong area of ​​Ningxia and sheep identification samples in Yichun City, Heilongjiang Province, Separation using the improved plate spreading method. During separation, the third-stage larvae of sheep nematodes are added to the plate as bait and hatched at 25°C. Check under an inverted microscope every day or every other day for 3 weeks to pick out a single predatory structure or prey Nematodes, transferred to 0.2% bran agar and cultured at 25°C for 3 to 14 days. After the conidia grew, pick a single conidia and put it on another 0.2% bran agar plate until a pure culture was obtained. . The morphology and data measurement of the conidia of the strain were obtained by the slide insertion method, and the DNA of the st...

Embodiment 3

[0057] (1) Preparation of nematode-predating fungus powder preparation

[0058] (1) Isolation, purification, isolation and identification of nematode-predating fungal isolates SDH035 and F088 From September 2013 to August 2014, samples from sheep manure piles in Wuzhong area of ​​Ningxia and sheep identification samples in Yichun City, Heilongjiang Province, Separation using the improved plate spreading method. During separation, add the third stage larvae of sheep nematodes as bait to the plate and incubate at 25°C. Check under an inverted microscope once a day or every other day for 3 weeks to pick out a single predatory structure or prey Nematodes, transferred to 0.2% bran agar and cultured at 25°C for 3 to 14 days. After the conidia grew, pick a single conidia and put it on another 0.2% bran agar plate until a pure culture was obtained. . The morphology and data measurement of the conidia of the strain were obtained by the slide insertion method, and the DNA of the strain...

technical field

[0001] The invention belongs to the technical field of microbiology, and in particular relates to a nematode-predating fungus powder preparation and its application. Background technique

[0002] Gastrointestinal nematode disease is the most common parasitic helminth disease in livestock, which is distributed worldwide and causes serious economic losses to the animal husbandry industry. For a long time, it has mainly relied on chemical anthelmintics to control gastrointestinal nematodes in livestock. With the continuous use of anthelmintics, the resistance of nematodes to anthelmintics has continued to increase, and it has caused environmental pollution and residues of chemical drugs in livestock products. At present, the resistance genes of animal parasitic nematodes to anthelmintics are widely spread in the breeding industry all over the world, which has caused a huge threat to animal husbandry production. People are actively looking for natural plant reso...

Examples

Embodiment 1

[0017] (1) Preparation of nematode-predating fungus powder preparation

[0018] (1) Isolation, purification, isolation and identification of nematode-predating fungal isolates SDH035 and F088 From September 2013 to August 2014, samples from sheep manure piles in Wuzhong area of ​​Ningxia and sheep identification samples in Yichun City, Heilongjiang Province, Separation using the improved plate spreading method. During separation, add the third stage larvae of sheep nematodes as bait to the plate and incubate at 25°C. Check under an inverted microscope once a day or every other day for 3 weeks to pick out a single predatory structure or prey Nematodes, transferred to 0.2% bran agar and cultured at 25°C for 3 to 14 days. After the conidia grew, pick a single conidia and put it on another 0.2% bran agar plate until a pure culture was obtained. . The morphology and data measurement of the conidia of the strain were obtained by the slide insertion method, and the DNA of the strain...

Embodiment 2

[0038] (1) Preparation of nematode-predating fungus powder preparation

[0039] (1) Isolation, purification, isolation and identification of nematode-predating fungal isolates SDH035 and F088 From September 2013 to August 2014, samples from sheep manure piles in Wuzhong area of ​​Ningxia and sheep identification samples in Yichun City, Heilongjiang Province, Separation using the improved plate spreading method. During separation, the third-stage larvae of sheep nematodes are added to the plate as bait and hatched at 25°C. Check under an inverted microscope every day or every other day for 3 weeks to pick out a single predatory structure or prey Nematodes, transferred to 0.2% bran agar and cultured at 25°C for 3 to 14 days. After the conidia grew, pick a single conidia and put it on another 0.2% bran agar plate until a pure culture was obtained. . The morphology and data measurement of the conidia of the strain were obtained by the slide insertion method, and the DNA of the st...

Embodiment 3

[0057] (1) Preparation of nematode-predating fungus powder preparation

[0058] (1) Isolation, purification, isolation and identification of nematode-predating fungal isolates SDH035 and F088 From September 2013 to August 2014, samples from sheep manure piles in Wuzhong area of ​​Ningxia and sheep identification samples in Yichun City, Heilongjiang Province, Separation using the improved plate spreading method. During separation, add the third stage larvae of sheep nematodes as bait to the plate and incubate at 25°C. Check under an inverted microscope once a day or every other day for 3 weeks to pick out a single predatory structure or prey Nematodes, transferred to 0.2% bran agar and cultured at 25°C for 3 to 14 days. After the conidia grew, pick a single conidia and put it on another 0.2% bran agar plate until a pure culture was obtained. . The morphology and data measurement of the conidia of the strain were obtained by the slide insertion method, and the DNA of the strain...

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