Establishment technology of hemsleya amabilis tissue culture system

Abstract

The invention discloses an establishment technology of a hemsleya amabilis tissue culture system. Hemsleya amabilis is a cucurbitaceous plant and tuberous roots are dried. The hemsleya amabilis is produced in Yunnan, Sichuan, Guizhou, Guangxi and the like. The hemsleya amabilis is dug when leaves become yellow in late autumn; over-ground parts and silt are removed; then the hemsleya amabilis is washed, sliced or not, and dried in the sun. The tuberous roots are of an irregular round shape or oval shape and are of a slight tortoise shape in the vertical direction; the diameter of the large tuberous roots can reach 20cm. Peels are brown yellow or grey brown and are slightly rough and certain peels have concave caudex traces; sections of the peels are light white or grey brown. The hemsleya amabilis is firm and is almost powdery. The hemsleya amabilis has slight odor and a slightly bitter taste. The reproduction speed and the scale are improved by utilizing a plane tissue culture technology, so that industrialized production of hemsleya amabilis germchits is realized. Hemsleya amabilis tissue culture regenerated plants are successfully obtained by taking stems with buds as explants through steps of inducing, reproducing, rooting, carrying out seedling exercising and transplanting and the like, and a systemic hemsleya amabilis tissue culture plant regeneration system is established. The reproduction coefficient is high, the seedling rate is high and the benefits are great.

Description

technical field

[0001] The invention belongs to the plant tissue culture category, and in particular relates to a technique for establishing a snow gall tissue culture system. Background technique

[0002] Snow gallbladder is a Cucurbitaceae plant with dry root tubers. Produced in Yunnan, Sichuan, Guizhou, Guangxi and other places. Xuedan is excavated at the end of autumn when the leaves are yellow, the above-ground part and the sediment are removed, washed, sliced ​​or not sliced, and dried in the sun. The roots are irregular round or oval, slightly longitudinally tortoise-shaped, and the largest diameter can reach 20cm. The outer skin is brownish yellow or grayish brown, slightly rough, and some have sunken stem base marks, and the cross section is pale white or grayish brown. The quality is firm and silty. Slight gas, slightly bitter taste. Commercial medicinal materials are often sliced ​​into round thick slices, slightly curled, 4-8mm thick, and 3-10cm in diameter....

Examples

Embodiment 1

[0016] (1) Disinfection of explants: Rinse the harvested snow gall stems with clear water for 10 minutes and gently brush off the impurities on them with a brush. Disinfect them with 75% ethanol solution in an ultra-clean workbench for 10 seconds, and then use sterile Wash 5 times with water, then rinse with 5% Antifoam solution for 6 minutes, rinse 5 times with sterile water, absorb the water with sterile filter paper and set aside.

[0017] (2) Induction culture: cut the sterilized snow gall stems in step (1) into about 1.5cm long stems according to the paired buds as a unit, cut off the browned part of the bottom of the stems during the treatment, and inoculate the induced Bud induction culture was carried out in the culture medium. After inoculation, they were first cultured in total darkness at 27°C for 4 days, then illuminated for 10 hours a day, with a light intensity of 1900lx and a culture temperature of 27°C until adventitious buds formed, and the induction rate was ...

Embodiment 2

[0022] (1) Disinfection of explants: Rinse the harvested snow gall stems with clean water for 9 minutes and gently brush off the impurities on them with a brush. Disinfect them with 75% ethanol solution in an ultra-clean workbench for 17 seconds, and then use sterile Wash 3 times with water, then rinse with 5% Antifoam solution for 5 minutes, rinse 3 times with sterile water, and then use sterile filter paper to absorb the water before use.

[0023] (2) Induction culture: cut the sterilized snow gall stems in step (1) into about 1.1cm long stems according to the paired buds as a unit, cut off the browned part of the bottom of the stems during the treatment, and inoculate them into the induced Bud induction culture was carried out in the culture medium. After inoculation, they were first cultured in total darkness at 26°C for 2 days, and then lighted for 9 hours a day with a light intensity of 2200lx and a culture temperature of 26°C until adventitious buds formed, and the indu...

technical field

[0001] The invention belongs to the plant tissue culture category, and in particular relates to a technique for establishing a snow gall tissue culture system. Background technique

[0002] Snow gallbladder is a Cucurbitaceae plant with dry root tubers. Produced in Yunnan, Sichuan, Guizhou, Guangxi and other places. Xuedan is excavated at the end of autumn when the leaves are yellow, the above-ground part and the sediment are removed, washed, sliced ​​or not sliced, and dried in the sun. The roots are irregular round or oval, slightly longitudinally tortoise-shaped, and the largest diameter can reach 20cm. The outer skin is brownish yellow or grayish brown, slightly rough, and some have sunken stem base marks, and the cross section is pale white or grayish brown. The quality is firm and silty. Slight gas, slightly bitter taste. Commercial medicinal materials are often sliced ​​into round thick slices, slightly curled, 4-8mm thick, and 3-10cm in diameter....

Examples

Embodiment 1

[0016] (1) Disinfection of explants: Rinse the harvested snow gall stems with clear water for 10 minutes and gently brush off the impurities on them with a brush. Disinfect them with 75% ethanol solution in an ultra-clean workbench for 10 seconds, and then use sterile Wash 5 times with water, then rinse with 5% Antifoam solution for 6 minutes, rinse 5 times with sterile water, absorb the water with sterile filter paper and set aside.

[0017] (2) Induction culture: cut the sterilized snow gall stems in step (1) into about 1.5cm long stems according to the paired buds as a unit, cut off the browned part of the bottom of the stems during the treatment, and inoculate the induced Bud induction culture was carried out in the culture medium. After inoculation, they were first cultured in total darkness at 27°C for 4 days, then illuminated for 10 hours a day, with a light intensity of 1900lx and a culture temperature of 27°C until adventitious buds formed, and the induction rate was ...

Embodiment 2

[0022] (1) Disinfection of explants: Rinse the harvested snow gall stems with clean water for 9 minutes and gently brush off the impurities on them with a brush. Disinfect them with 75% ethanol solution in an ultra-clean workbench for 17 seconds, and then use sterile Wash 3 times with water, then rinse with 5% Antifoam solution for 5 minutes, rinse 3 times with sterile water, and then use sterile filter paper to absorb the water before use.

[0023] (2) Induction culture: cut the sterilized snow gall stems in step (1) into about 1.1cm long stems according to the paired buds as a unit, cut off the browned part of the bottom of the stems during the treatment, and inoculate them into the induced Bud induction culture was carried out in the culture medium. After inoculation, they were first cultured in total darkness at 26°C for 2 days, and then lighted for 9 hours a day with a light intensity of 2200lx and a culture temperature of 26°C until adventitious buds formed, and the indu...