Blocking support frame and preparation method thereof

Abstract

The invention provides a preparation method of a blocking support frame. The method comprises the following steps of (A) inoculating a solid substrate with a microbial strain; after fermentation, obtaining a solid substrate with a bacterial cellulose membrane on the surface, wherein the microbial strain is a strain capable of generating bacterial cellulose; (B) putting the solid substrate obtainedin the step (A) into aerosol containing nutrition solution; performing fermentation; (C) putting a metal support frame onto the surface of the solid substrate obtained in the step (B) to obtain a combined support frame; (D) putting the combined support frame into the aerosol containing nutrition solution; performing fermentation to obtain a blocking support frame. The preparation method of the blocking support frame provided by the invention can be used for preparing and obtaining the clinic support frame material with a good blocking function; growth factors can pass through the material while cells and bacteria can be blocked; good biocompatibility is realized; the blocking support frame belongs to an ideal clinic blocking support frame.

Description

technical field

[0001] The invention relates to the technical field of composite materials, in particular to a barrier bracket and a preparation method thereof. Background technique

[0002] Bone tissue is one of the few tissues and organs with self-regeneration ability. This regeneration ability not only accompanies individual bone development and bone tissue damage repair process, but can still work effectively even after the individual matures.

[0003] Guided Tissue Regeneration (GTR) is a surgical method to place a physical barrier to selectively separate different tissues. After 20 years of exploration, guided tissue regeneration has been established, and the basic concept of guided bone regeneration has been derived from it, that is, to use the shielding effect of the barrier membrane to separate the surrounding soft tissue from the bone surface of the wound area, such as preventing the growth of the calcaneus. Fibroblasts grow into fibrous connective tissue, allowin...

Examples

preparation example Construction

[0033] Aiming at the characteristics of the barrier stent in the prior art, the present application provides a preparation method of the barrier stent. The barrier stent is essentially a barrier stent based on bacterial fermentation to produce a bacterial cellulose film and filled with a metal stent. The barrier stent has good Its barrier function can block bacteria and cells while permeating growth factors, and has good biocompatibility. It is an ideal clinical barrier scaffold. Specifically, the preparation method of the barrier stent of the present invention comprises the following steps:

[0034] A) microbial strains are inoculated onto a solid substrate, and a solid substrate having a bacterial cellulose film on the surface is obtained after fermentation; the microbial strains are bacterial strains that can produce bacterial cellulose;

[0035] B) placing the solid substrate obtained in step A) in an aerosol containing nutrient solution, and fermenting;

[0036] C) placi...

Embodiment 1

[0050] Prepare 500mL aqueous solution containing 10wt% glucose, 1wt% yeast powder, 2wt% calcium carbonate, and 1.5wt% agar, stir to dissolve and boil, divide into five 250mL beakers, seal the beakers with tissue culture film, and autoclave Sterilize the pot, cool and stand to obtain a solid substrate; prepare an aqueous solution containing 5wt% glucose and 1wt% yeast powder as a nutrient solution; prepare a metal titanium mesh and sterilize it for use;

[0051] Inoculate the strain of Gluconacetobacter xylinum on the above solid substrate, put it into a constant temperature incubator, and cultivate it at 28°C for 24h;

[0052] The solid substrate obtained above is transferred from the constant temperature incubator to the aerosol spray formed by the nutrient solution, and the spray speed of the nutrient solution is adjusted to 2mL / hour;

[0053] After the above-mentioned process was fermented for 2 days, a sterilized titanium mesh was placed on the surface of the formed bacter...

Embodiment 2

[0058] Prepare 500mL aqueous solution containing 10wt% glucose, 1wt% yeast powder, 2wt% calcium carbonate, and 1.5wt% agar, stir to dissolve and boil, sterilize in an autoclave, pour into a square shallow dish with a side length of 40cm, cool and stand Obtain a solid substrate; prepare an aqueous solution containing 5wt% glucose and 1wt% yeast powder as a nutrient solution; prepare a 3 mg / mL nano-hydroxyapatite solution with sterile water; prepare a metal titanium mesh and sterilize it for use;

[0059] Inoculate the strain of Gluconacetobacter xylinum on the above solid substrate, put it into a constant temperature incubator, and cultivate it at 28°C for 24h;

[0060] The solid substrate obtained above is transferred from the constant temperature incubator to the aerosol spray formed by the nutrient solution and the nano-hydroxyapatite solution, and the spray speed of the two solutions is adjusted to 2mL / hour;

[0061] After the above-mentioned process was fermented for 3 day...

technical field

[0001] The invention relates to the technical field of composite materials, in particular to a barrier bracket and a preparation method thereof. Background technique

[0002] Bone tissue is one of the few tissues and organs with self-regeneration ability. This regeneration ability not only accompanies individual bone development and bone tissue damage repair process, but can still work effectively even after the individual matures.

[0003] Guided Tissue Regeneration (GTR) is a surgical method to place a physical barrier to selectively separate different tissues. After 20 years of exploration, guided tissue regeneration has been established, and the basic concept of guided bone regeneration has been derived from it, that is, to use the shielding effect of the barrier membrane to separate the surrounding soft tissue from the bone surface of the wound area, such as preventing the growth of the calcaneus. Fibroblasts grow into fibrous connective tissue, allowin...

Examples

preparation example Construction

[0033] Aiming at the characteristics of the barrier stent in the prior art, the present application provides a preparation method of the barrier stent. The barrier stent is essentially a barrier stent based on bacterial fermentation to produce a bacterial cellulose film and filled with a metal stent. The barrier stent has good Its barrier function can block bacteria and cells while permeating growth factors, and has good biocompatibility. It is an ideal clinical barrier scaffold. Specifically, the preparation method of the barrier stent of the present invention comprises the following steps:

[0034] A) microbial strains are inoculated onto a solid substrate, and a solid substrate having a bacterial cellulose film on the surface is obtained after fermentation; the microbial strains are bacterial strains that can produce bacterial cellulose;

[0035] B) placing the solid substrate obtained in step A) in an aerosol containing nutrient solution, and fermenting;

[0036] C) placi...

Embodiment 1

[0050] Prepare 500mL aqueous solution containing 10wt% glucose, 1wt% yeast powder, 2wt% calcium carbonate, and 1.5wt% agar, stir to dissolve and boil, divide into five 250mL beakers, seal the beakers with tissue culture film, and autoclave Sterilize the pot, cool and stand to obtain a solid substrate; prepare an aqueous solution containing 5wt% glucose and 1wt% yeast powder as a nutrient solution; prepare a metal titanium mesh and sterilize it for use;

[0051] Inoculate the strain of Gluconacetobacter xylinum on the above solid substrate, put it into a constant temperature incubator, and cultivate it at 28°C for 24h;

[0052] The solid substrate obtained above is transferred from the constant temperature incubator to the aerosol spray formed by the nutrient solution, and the spray speed of the nutrient solution is adjusted to 2mL / hour;

[0053] After the above-mentioned process was fermented for 2 days, a sterilized titanium mesh was placed on the surface of the formed bacter...

Embodiment 2

[0058] Prepare 500mL aqueous solution containing 10wt% glucose, 1wt% yeast powder, 2wt% calcium carbonate, and 1.5wt% agar, stir to dissolve and boil, sterilize in an autoclave, pour into a square shallow dish with a side length of 40cm, cool and stand Obtain a solid substrate; prepare an aqueous solution containing 5wt% glucose and 1wt% yeast powder as a nutrient solution; prepare a 3 mg / mL nano-hydroxyapatite solution with sterile water; prepare a metal titanium mesh and sterilize it for use;

[0059] Inoculate the strain of Gluconacetobacter xylinum on the above solid substrate, put it into a constant temperature incubator, and cultivate it at 28°C for 24h;

[0060] The solid substrate obtained above is transferred from the constant temperature incubator to the aerosol spray formed by the nutrient solution and the nano-hydroxyapatite solution, and the spray speed of the two solutions is adjusted to 2mL / hour;

[0061] After the above-mentioned process was fermented for 3 day...

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