A kind of synthetic method of c-glycoside derivative

Abstract

The invention relates to the technical field of medicinal chemistry, and specifically discloses a synthesis method of C-glycoside derivatives. Add the compound of formula (II) and triethylsilane into dichloromethane, under nitrogen protection, cool down in a dry-ice acetonitrile bath to -35°C~-45°C, add 20% boron trifluoride acetonitrile complex dropwise, at -35°C~ Carry out the synthesis reaction of NaC-glycoside derivatives shown in formula (I) by incubating at -45°C; after that, add the reaction system dropwise into aqueous sodium bicarbonate solution to quench the reaction. The invention improves the way of quenching reaction in the existing synthesis process, can quickly quench the reaction, has short quenching time, is easy to operate, and can effectively avoid the problem of impurity III growth caused by the temperature rise of the reaction system.

Description

technical field

[0001] The invention relates to the technical field of medicinal chemistry, in particular to a method for synthesizing C-glycoside derivatives. Background technique

[0002] Hyperglycemia is considered a major risk factor for the development of diabetic complications and may be directly related to impaired insulin secretion in advanced type II diabetes. Normalization of insulin can therefore be expected to improve blood glucose in type 2 diabetic patients. Most of the existing diabetes drugs are insulin secretory drugs or insulin sensitizers, such as sulfonylureas, glinides, thiazolidinediones, and metformin, which have potential side effects, such as easy to cause weight gain , hypoglycemia, lactic acidosis, etc. Therefore, there is an urgent need to develop novel, safe and effective antidiabetic drugs with a mechanism of action.

[0003] In the kidney, glucose is freely filtered from the glomerulus (approximately 180 g / day), but is almost reabsorbed by ac...

Examples

Embodiment 1

[0049] This example is used to illustrate the method of synthesizing and purifying the compound of formula (I) starting from the compound of formula (II), and further synthesizing the L-proline co-crystal complex of the compound of formula (I).

[0050] 1. Under the protection of nitrogen, add dichloromethane (364.1 kg) into a 500 L cryogenic reaction kettle, start stirring, and add triethylsilane (20.3 kg) and compound of formula (II) (28.1 kg). Cool down to -40°C. 20% boron trifluoride acetonitrile complex (69.1 kg) was added dropwise under temperature control for about 2 hours. After dropping, keep warm for 3 hours to synthesize the compound of formula (I).

[0051] 2. Add 5% sodium bicarbonate aqueous solution (313.73kg) into the 1000 L cryogenic reaction kettle. The feed liquid in the 500 L cryogenic reactor kept at -40°C was directly dropped into the 1000 L cryogenic reactor to quench the reaction (dropping for about 1 hour), and the temperature in the 1000 L cryogenic...

Embodiment 2

[0059] In this example, the content of impurity (X) in the L-proline eutectic complex of the compound of formula (I) prepared in Example 1 was detected by HPLC.

[0060] The detection method is as follows:

[0061] Reagent solution: diammonium hydrogen phosphate, phosphoric acid, methanol, acetonitrile, ultrapure water.

[0062] Instruments and equipment: electronic balance, high performance liquid chromatography, pH meter, centrifuge.

[0063] Chromatographic conditions: determined according to high-performance liquid chromatography (Chinese Pharmacopoeia 2020 Edition Sibu General Rules 0512), using octadecylsilane-bonded silica gel as a filler column (Agilent Eclipse XDB-C18, 150mm×4.6mm, 5μm); Mobile phase A is 0.01mol / L diammonium hydrogen phosphate solution (take 1.32g of diammonium hydrogen phosphate, add 1000mL of water to ultrasonically dissolve, adjust the pH value to 5.00±0.05 with phosphoric acid)-acetonitrile (90:10), mobile phase B is Water-acetonitrile (10:90);...

Embodiment 3

[0068] In this example, according to the synthesis method described in Example 1, three batches of samples were respectively prepared with different amounts of starting materials, and the samples were subjected to high-efficiency liquid phase detection, and the results are as follows:

[0069]

[0070] Experimental results prove that the synthetic method provided by the present invention has good repeatability.

technical field

[0001] The invention relates to the technical field of medicinal chemistry, in particular to a method for synthesizing C-glycoside derivatives. Background technique

[0002] Hyperglycemia is considered a major risk factor for the development of diabetic complications and may be directly related to impaired insulin secretion in advanced type II diabetes. Normalization of insulin can therefore be expected to improve blood glucose in type 2 diabetic patients. Most of the existing diabetes drugs are insulin secretory drugs or insulin sensitizers, such as sulfonylureas, glinides, thiazolidinediones, and metformin, which have potential side effects, such as easy to cause weight gain , hypoglycemia, lactic acidosis, etc. Therefore, there is an urgent need to develop novel, safe and effective antidiabetic drugs with a mechanism of action.

[0003] In the kidney, glucose is freely filtered from the glomerulus (approximately 180 g / day), but is almost reabsorbed by ac...

Examples

Embodiment 1

[0049] This example is used to illustrate the method of synthesizing and purifying the compound of formula (I) starting from the compound of formula (II), and further synthesizing the L-proline co-crystal complex of the compound of formula (I).

[0050] 1. Under the protection of nitrogen, add dichloromethane (364.1 kg) into a 500 L cryogenic reaction kettle, start stirring, and add triethylsilane (20.3 kg) and compound of formula (II) (28.1 kg). Cool down to -40°C. 20% boron trifluoride acetonitrile complex (69.1 kg) was added dropwise under temperature control for about 2 hours. After dropping, keep warm for 3 hours to synthesize the compound of formula (I).

[0051] 2. Add 5% sodium bicarbonate aqueous solution (313.73kg) into the 1000 L cryogenic reaction kettle. The feed liquid in the 500 L cryogenic reactor kept at -40°C was directly dropped into the 1000 L cryogenic reactor to quench the reaction (dropping for about 1 hour), and the temperature in the 1000 L cryogenic...

Embodiment 2

[0059] In this example, the content of impurity (X) in the L-proline eutectic complex of the compound of formula (I) prepared in Example 1 was detected by HPLC.

[0060] The detection method is as follows:

[0061] Reagent solution: diammonium hydrogen phosphate, phosphoric acid, methanol, acetonitrile, ultrapure water.

[0062] Instruments and equipment: electronic balance, high performance liquid chromatography, pH meter, centrifuge.

[0063] Chromatographic conditions: determined according to high-performance liquid chromatography (Chinese Pharmacopoeia 2020 Edition Sibu General Rules 0512), using octadecylsilane-bonded silica gel as a filler column (Agilent Eclipse XDB-C18, 150mm×4.6mm, 5μm); Mobile phase A is 0.01mol / L diammonium hydrogen phosphate solution (take 1.32g of diammonium hydrogen phosphate, add 1000mL of water to ultrasonically dissolve, adjust the pH value to 5.00±0.05 with phosphoric acid)-acetonitrile (90:10), mobile phase B is Water-acetonitrile (10:90);...

Embodiment 3

[0068] In this example, according to the synthesis method described in Example 1, three batches of samples were respectively prepared with different amounts of starting materials, and the samples were subjected to high-efficiency liquid phase detection, and the results are as follows:

[0069]

[0070] Experimental results prove that the synthetic method provided by the present invention has good repeatability.

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