The present invention provides a GFAP, NFL and UCH-L1 combined detection kit, comprising: an acridinium ester labeled
antibody working solution and a
magnetic bead coated
antibody working solution; the acridinium ester labeled
antibody working solution contains acridinium ester labeled GFAP, NFL and UCH-L1 antibodies, and the
magnetic bead coated antibody working solution contains
magnetic bead coated GFAP, NFL and UCH-L1 antibodies. By screening the components and the content of each component of the acridinium ester labeled working solution and the magnetic bead labeled working solution, a stable
working environment can be provided for the acridinium ester labeled GFAP, NFL and UCH-L1 antibodies and the magnetic beads labeled with the GFAP, NFL and UCH-L1 antibodies, and specific recognition and detection of GFAP, NFL and UCH-L1 are achieved, thereby realizing the triple detection of GFAP, NFL and UCH-L1 for early diagnosis of patients with
brain trauma.