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Process of regenerating internal cell coenzyme NADPH by pentose and its application

A technology for coenzyme regeneration, pentose sugar, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc.

Active Publication Date: 2009-09-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the method of using carbohydrates as auxiliary substrates to regenerate coenzyme NADPH in whole cells, hexose sugars such as glucose are usually used as auxiliary substrates, but there is no report on the method of regenerating intracellular coenzyme NADPH using pentose sugars.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Resuspend 2 g of Candida parapsilosis wet cells that have catalyzed a transformation reaction in 20 ml of 100 mmol / L potassium phosphate buffer solution (pH6.5), add 300 mg of racemic phenylethylene glycol, 40 mg of xylose , Reacted at 30°C, 150r / min for 48h. After the reaction, the mixture was centrifuged, and the supernatant was taken for extraction. The optical purity of the product (S)-PED was 91.53% e.e., and the yield was 80.41%.

Embodiment 2

[0049] Resuspend 2 g of Candida parapsilosis wet cells that have catalyzed a conversion reaction in 20 ml of 100 mmol / L potassium phosphate buffer solution (pH6.5), add 300 mg of racemic phenylethylene glycol, 400 mg of xylose , Reacted at 30°C, 150r / min for 48h. After the reaction, the mixture was centrifuged, and the supernatant was taken for extraction. The optical purity of the product (S)-PED was 93.12% e.e., and the yield was 82.19%.

Embodiment 3

[0051] Resuspend 2 g of Candida parapsilosis wet cells that have catalyzed a conversion reaction in 20 ml of 100 mmol / L potassium phosphate buffer solution (pH6.5), add 300 mg of racemic phenyl glycol, 160 mg of xylose , Reacted at 30°C, 150r / min for 48h. After the reaction, the mixture was centrifuged, and the supernatant was taken for extraction. The optical purity of the product (S)-PED was 98.72% e.e., and the yield was 86.43%.

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Abstract

The present invention is the process of regenerating intracellular coenzyme NADPH with pentose and its application, and belongs to the field of biologically catalyzed asymmetrical conversion technology. Into the conversion reaction system, pentose as the auxiliary substrate for regenerating the coenzyme is added to raise the stereo selective conversion capacity of thallus. Adding pentose in 8 mg / ml can raise the enantiomeric excess value of the product (S)-phenyl ethylene glycol from 86 % to 98 % and the yield from 77 % to 85 %, and makes it possible to use the thallus for several times. The process regenerates great amount of NADPH and replenishes coenzyme for the conversion reaction. The present invention is helpful for understanding the cell metabolism mechanism related to coenzyme regeneration and is significant to the development of intracellular coenzyme regenerating system.

Description

technical field [0001] The invention relates to a method for regenerating intracellular coenzyme NADPH with pentose sugar and its application, belonging to the technical field of biocatalytic asymmetric transformation. Background technique [0002] Chiral compounds play an important role in people's lives. Since the two enantiomers are different in pharmacology, toxicology and functional effects, the preparation of optically pure chiral modular compounds is very important in medicine, agriculture, materials and Environmental protection and other fields are of great significance. [0003] At present, the common methods for the resolution of racemic compounds in the world mainly include: chemical resolution, chromatographic resolution, liquid membrane resolution or membrane resolution of chiral solid membrane resolution, and biological methods. The preparation of optically pure chiral compounds by biological transformation has the advantages of mild reaction conditions, singl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/36C12P7/18C12P41/00C12R1/72
CPCY02P20/582
Inventor 徐岩聂尧吕腾飞
Owner JIANGNAN UNIV
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