Medicine health-care usage of globefish I collagen extraction and preparing process thereof
一种胶原蛋白、提取物的技术，应用在河鲀Ⅰ型胶原蛋白提取物的医药保健用途及其制备工艺领域，能够解决副作用、患者生理和心理伤害等问题
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Embodiment 1
[0147] Example 1: Take 100 grams of puffer fish skin, add 500 ml of deionized water, heat and extract at 100 ° C for 8 hours, homogenize, and after the homogenate is clarified by high-speed centrifugation, add NaCl to the supernatant to a final concentration of 0.2 mol / L , passed through a DEAE-Sepharose fastflow column, eluted with 0.2mol / L NaCl solution, the eluate was desalted and concentrated by ultrafiltration, and the concentrated solution was spray-dried to obtain dry powder of pufferfish type I collagen extract. The isoelectric points of the two subunits of pufferfish type I collagen measured by isoelectric focusing were α1(I): 4.85±0.5 and α2(I): 6.71±0.5 (see Figure 1). After being hydrolyzed with trifluoroacetic acid, the protein-binding sugar content of the puffer fish type I collagen extract was determined by the o-toluidine method to be 1.16%.
Embodiment 2
[0148] Embodiment 2: get puffer fish bone (fin) 500 grams, add deionized water 1500ml, after 10 hours of heating extraction at 100 ℃, filter, filtrate vacuum concentration to 200ml, add HCl to final concentration and be 0.2mol / L, 60 After hydrolysis at ℃ for 2 hours, the hydrolyzate was concentrated and deacidified, centrifuged and clarified, and the centrifuged supernatant was adjusted to pH 7.4, then added NaCl to 0.18mol / L, passed through a DEAE-cellulose column, eluted with 0.2mol / L NaCl solution, and Deliquification, ultrafiltration, desalination, concentration, spray drying to obtain 47.2 g of light yellow pufferfish type I collagen extract dry powder.
Embodiment 3
[0149] Embodiment 3: get puffer fish skin 100 grams, add deionized water 1000ml, at 110 ℃, under 2 atmospheric pressures, heat extraction 60 minutes, homogenate, homogenate liquid is vacuum-concentrated to 150ml, adds glacial acetic acid to final concentration of 0.8mol / L, hydrolysis at 45°C for 4 hours. The hydrolyzate was clarified by centrifugation, and spray-dried to obtain 23 grams of dry powder of the light yellow pufferfish type I collagen extract. As determined by the Kivirikko method, its collagen content is 75.92%. Its total protein content was 86.22% as determined by the Kjeldahl nitrogen method.
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