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Medicine health-care usage of globefish I collagen extraction and preparing process thereof

一种胶原蛋白、提取物的技术，应用在河鲀Ⅰ型胶原蛋白提取物的医药保健用途及其制备工艺领域，能够解决副作用、患者生理和心理伤害等问题

Inactive Publication Date: 2010-01-27
NANJING BESSON PHARMACY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0024] One of the biggest problems of clinical chemotherapy is the side effects after chemotherapy (such as severe gastrointestinal reactions, weight loss, decreased immunity and leukopenia, etc.), which often cause huge physical and psychological harm to patients, which sometimes even exceeds damage caused by the tumor itself

Method used

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  • Medicine health-care usage of globefish I collagen extraction and preparing process thereof
  • Medicine health-care usage of globefish I collagen extraction and preparing process thereof
  • Medicine health-care usage of globefish I collagen extraction and preparing process thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0147] Example 1: Take 100 grams of puffer fish skin, add 500 ml of deionized water, heat and extract at 100 ° C for 8 hours, homogenize, and after the homogenate is clarified by high-speed centrifugation, add NaCl to the supernatant to a final concentration of 0.2 mol / L , passed through a DEAE-Sepharose fastflow column, eluted with 0.2mol / L NaCl solution, the eluate was desalted and concentrated by ultrafiltration, and the concentrated solution was spray-dried to obtain dry powder of pufferfish type I collagen extract. The isoelectric points of the two subunits of pufferfish type I collagen measured by isoelectric focusing were α1(I): 4.85±0.5 and α2(I): 6.71±0.5 (see Figure 1). After being hydrolyzed with trifluoroacetic acid, the protein-binding sugar content of the puffer fish type I collagen extract was determined by the o-toluidine method to be 1.16%.

Embodiment 2

[0148] Embodiment 2: get puffer fish bone (fin) 500 grams, add deionized water 1500ml, after 10 hours of heating extraction at 100 ℃, filter, filtrate vacuum concentration to 200ml, add HCl to final concentration and be 0.2mol / L, 60 After hydrolysis at ℃ for 2 hours, the hydrolyzate was concentrated and deacidified, centrifuged and clarified, and the centrifuged supernatant was adjusted to pH 7.4, then added NaCl to 0.18mol / L, passed through a DEAE-cellulose column, eluted with 0.2mol / L NaCl solution, and Deliquification, ultrafiltration, desalination, concentration, spray drying to obtain 47.2 g of light yellow pufferfish type I collagen extract dry powder.

Embodiment 3

[0149] Embodiment 3: get puffer fish skin 100 grams, add deionized water 1000ml, at 110 ℃, under 2 atmospheric pressures, heat extraction 60 minutes, homogenate, homogenate liquid is vacuum-concentrated to 150ml, adds glacial acetic acid to final concentration of 0.8mol / L, hydrolysis at 45°C for 4 hours. The hydrolyzate was clarified by centrifugation, and spray-dried to obtain 23 grams of dry powder of the light yellow pufferfish type I collagen extract. As determined by the Kivirikko method, its collagen content is 75.92%. Its total protein content was 86.22% as determined by the Kjeldahl nitrogen method.

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Abstract

The invention relates to a medicine health-care usage of globefish I collagen extraction. The main chemical components and active components in the globefish I collagen extraction are: globefish natural unmodified I collagen or globefish modified I collagen and a part of hydrolysate thereof. The invention also relates to a preparing process, an immunology measuring method thereof and the application in the treatment and health care as the effective component.

Description

technical field [0001] The present invention relates to the application of puffer fish type I collagen extract as an active ingredient in the preparation of medicines and health food for the treatment and prevention of the following diseases. The main chemical and active ingredients of puffer fish type I collagen extract are natural Denatured type I collagen or puffer puffer denatured type I collagen and its partial hydrolyzate, the present invention also relates to the preparation process, immunological assay method of said puffer fish type I collagen extract and its use as an active ingredient in treatment and health care use in . Background technique [0002] According to fish taxonomy, "pufferfish" used in the present invention belongs to Tetrodontiformes (Tetrodontiformes) Tetrodontidae, and pufferfish is also called bubble fish (pufferfish, Swellfish, Balloonfish, Blowfish). But today, due to various historical reasons and customs, puffer fish are often mistakenly cal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/60A61P1/00A61P17/00A61P19/02A61P35/00A61K38/39A61P1/04A61P1/16A61P25/32A61P37/02C07K14/78C12P21/00
CPCA23L1/05625A61K38/39A23L29/284A61P1/00A61P1/04A61P1/12A61P1/14A61P1/16A61P9/04A61P13/12A61P17/00A61P19/02A61P25/02A61P25/32A61P29/00A61P35/00A61P35/02A61P35/04A61P37/02A61P37/04
Inventor 陈丁丁高利忠
Owner NANJING BESSON PHARMACY
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