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Method for synchronous improvement of hybrid rape parents

A parent and rapeseed technology, applied in plant genetic improvement, botanical equipment and methods, angiosperms/flowering plants, etc., can solve the problems of lack of mutual selection and improvement of parental groups, and achieve the effect of improving efficiency and improving effect

Inactive Publication Date: 2010-12-29
江西省农业科学院旱作物研究所
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  • Summary
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  • Application Information

AI Technical Summary

Problems solved by technology

However, for self-pollination and cross-pollination crops, it is currently limited to the improvement of a single population, and the male sterile line is mainly used to solve the difficulty of artificial intercrossing. So far, there is still a lack of effective technology to carry out mutual rotation improvement between parent populations

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Screening of parental samples

[0037] G5034 is a double-low recessive male sterile line, and R210 is a double-low quality sterile restorer line. The combination of the two has a strong advantage. The candidate parents were selected to cross with G5034 and R210, the special combining ability was analyzed, and the candidate parents were divided into two groups. At the same time, molecular marker technology was used to analyze the SSR band pattern of each candidate parent, and to screen two parent samples clustered with G5034 and R210. Based on the results of molecular analysis and special combining ability analysis, two parent samples involved in the construction of the basic population were determined: sterile line parent sample (SP) and restorer line parent sample (RP);

[0038] 2. Construction of basic groups

[0039] (1) Construction of the basic population of recessive GMS lines: each parent of the SP sample was crossed with G5034, F1 was self-crossed, the ster...

Embodiment 2

[0047] 1. Screening of parental samples

[0048] With embodiment 1.

[0049] 2. Construction of basic groups

[0050] (1) Construction of the basic population of recessive nuclear sterility: the same as in Example 1.

[0051] (2) Construction of dominant genic male sterile basic population: each parent of the RP sample was crossed with D5226 (double low homozygous dominant genic male sterile line), and F1 sterile plants (parents were Temporary line) were selected and corresponding Parents are backcrossed or fertile plants (parents are restorer lines) are selfed, each line is mixed with the same amount of backcrossed or selfed seeds, open pollination for one generation under isolation conditions, and the seeds of sterile plants are collected. After quality analysis, 30- 40 plants were mixed with the same amount of seeds to construct the basic population of dominant genital sterility (DCO).

[0052] 3. Mutual reincarnation selection technical process

[0053] With embodiment...

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Abstract

The invention relates to a method for synchronously improving the parents of hybrid rape, based on present advanced parents and two selected parents to be transferred hidden corn sterility system and dominant sterility system, while the invention uses two sterility systems to process alternative selection that selecting parental sample, building base group, and selecting. The invention can hold the genetic heterogeneity of parents, and collect more useful gene points and alleles in two groups, to improve the advantages of parents. The invention provides a method based on gene heterogeneity molecule check and special match force analysis based on present advanced parents to build two turned selected base groups of parental samples, to improve the selection effect.

Description

technical field [0001] The invention belongs to the field of rape variety breeding and relates to a method for synchronously improving the parents of hybrid rapeseed. Background technique [0002] Utilization of heterosis is an effective technical approach to increase crop yield, enhance stress resistance, and alleviate the contradiction between quality and yield, and is an important field of crop genetic improvement. The comprehensive performance and genetic differences of the parents are the basis of heterosis. Aggregating more excellent genes and alleles in the parents and coordinating the interaction between genes and alleles have always been the main direction of genetic improvement. There are two commonly used methods for parental improvement. One is for quality traits or traits controlled by major genes, using line selection, radiation, chemical or aerospace mutagenesis, transgenic and hybridization, backcross breeding, etc., but only for individual or a few The impr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04A01H5/00
Inventor 宋来强邹晓芬邹小云张建模熊任香
Owner 江西省农业科学院旱作物研究所
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