Immunosuppressive exosomes

A technology of exosomes and immune response, which is applied in the direction of antibody medical components, extracellular fluid diseases, medical preparations containing active ingredients, etc., and can solve problems such as enhanced immune stimulation

Inactive Publication Date: 2007-08-22
UNIVERSITY OF PITTSBURGH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] However, this approach also has some potential problems
For example, although administration of immature hyporesponsive DCs can enhance tolerogenicity in the host, transduction of DCs by viral-based vectors may stimulate their maturation, resulting in enhanced immunostimulatory capacity (Rea et al., 1999, J. Virol .73:10245-10253)
Thus, despite their potential efficacy in slowing disease onset, administration of intact dendritic cells may have unwanted consequences

Method used

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Embodiment Construction

[0059] For clarity but not limitation, the Detailed Description of the Invention is divided into the following subsections:

[0060] (i) the cellular source of the exosome;

[0061] (ii) conditioning the cells to harvest exosomes;

[0062] (iii) preparing exosomes from the cells;

[0063] (iv) preparing exosomes from serum;

[0064] (v) compositions comprising exosomes;

[0065] (vi) immunosuppressive methods; and

[0066] (vii) A method of antagonizing exosome-mediated immunosuppression.

[0067] 5.1 Cellular source of exosomes

[0068] The exosomes of the present invention can be derived from a variety of different cells, including but not limited to antigen presenting cells ("APC") such as dendritic cells ("DC") and macrophages, which can be obtained from, for example, bone marrow, spleen, lymph nodes or Tissues such as the thymus, or harvested from the peripheral blood or serum from which they were derived. Also included within the scope of the invention are special...

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Abstract

The present invention relates to methods and compositions for use in mediating an immunosuppressive reaction. The compositions of the invention comprise exosomes having immunosuppressive activity. Such exosomes may be derived from a variety of different cell types, including antigen presenting cells such as dendritic cells and macrophages. Prior to isolation of exosomes, the cells may be genetically engineered to express molecules capable of enhancing the immunosuppressive activity of said exosomes and / or may be exposed to one or more agents, such as cytokines or cytokine inhibitors, which are also capable of enhancing the immunosuppressive activity of exosomes. The present invention also relates to the use of such exosomes for the treatment of diseases and disorders associated with undesirable activation of the immune system. The present invention also includes exosomes isolated directly from serum that have been shown to be immunosuppressive.

Description

[0001] describe [0002] 1 Introduction [0003] The present invention relates to methods and compositions for mediating immunosuppressive responses. The compositions of the invention comprise exosomes having immunosuppressive activity. Such exosomes can be derived from a variety of different cell types, including antigen-presenting cells such as dendritic cells and macrophages. Isolation of exosomes may also be accomplished by genetically engineering cells to express molecules that enhance the immunosuppressive activity of said exosomes, and / or by contacting cells with one or more agents, such as cytokines or cytokine inhibitors. Can enhance the immunosuppressive activity of exosomes. The invention also relates to the use of such exosomes in the treatment of diseases and conditions associated with unwanted activation of the immune system. The invention also encompasses the direct isolation of exosomes from serum that has been shown to be immunosuppressed. 2. Background of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00C12N5/00A61K39/38C12N5/02A61K35/15A61K35/16
CPCA61K35/16A61K2039/55555A61K35/15A61K39/0008A61P1/16A61P3/10A61P5/14A61P7/04A61P7/06A61P9/00A61P11/06A61P17/02A61P17/06A61P17/12A61P17/14A61P19/02A61P19/08A61P21/04A61P25/28A61P29/00A61P35/00A61P37/06A61P37/08A61K38/17A61K39/38C12N5/00
Inventor P·D·罗宾斯金善嬉P·韦林
Owner UNIVERSITY OF PITTSBURGH
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