Novel finger print developing reagent and developing method thereof
A fingerprint and reagent technology, applied in measuring devices, material analysis through optical means, instruments, etc., can solve the problems of high DFO price and environmental pollution, and achieve the effect of mature synthesis technology, high sensitivity and high yield
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[0023] The present invention uses p-2,3,7-trihydroxy-9-(4'-nitro)phenylfluorescein, namely p-nitrophenylfluorescein (p-NO 2 -PF) as a fingerprint developer, its structural formula is:
[0024]
[0025] The preparation method of the present invention is as follows:
[0026] Triglycerol triacetate was synthesized from p-phenol by a classical method, and then condensed with p-benzaldehyde in a sulfuric acid medium, and then hydrolyzed to obtain p-NO 2 -PF, its synthesis steps are as follows:
[0027] Add the acetic acid aqueous solution of chromium trioxide dropwise to the acetic acid aqueous solution of p-phenol and react at 15 ° C to obtain p-benzoquinone; then add p-benzoquinone in batches to acetic anhydride (sulfuric acid is an acidic medium) and react at below 50 ° C. Obtain trimemol triacetate; add p-nitrobenzaldehyde in batches to the ethanol solution of trimemol triacetate (using sulfuric acid as the acidic medium) to react at 75°C, and finally hydrolyze the solid o...
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