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Reagent for sample analysis, kit for sample analysis and method for sample analysis

A technology for reagents and surfactants, applied in the field of sample analysis reagents, sample analysis kits and sample analysis

Active Publication Date: 2010-09-29
SYSMEX CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009]However, with the above-mentioned old method, in samples with a large number of white blood cells, there may sometimes be a problem that basophils and white blood cells other than basophils are not easily separated

Method used

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  • Reagent for sample analysis, kit for sample analysis and method for sample analysis
  • Reagent for sample analysis, kit for sample analysis and method for sample analysis
  • Reagent for sample analysis, kit for sample analysis and method for sample analysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0097] The fluorochromes used in the following examples are as follows:

[0098] NK—1840

[0099] [chemical 12]

[0100]

[0101] NK—2929

[0102] [chemical 13]

[0103]

[0104] NK-3375

[0105] [chemical 14]

[0106]

[0107] NK-3662

[0108] [chemical 15]

[0109]

[0110] NK-5056

[0111] [chemical 16]

[0112]

[0113] NK-9001

[0114] [chemical 17]

[0115]

[0116] NK-9002

[0117] [chemical 18]

[0118]

[0119] NK-9003

[0120] [chemical 19]

[0121]

[0122] NK-4249

[0123] [chemical 20]

[0124]

[0125] NK-3606

[0126] [chem 21]

[0127]

[0128] NK-3620

[0129] [chem 22]

[0130]

Embodiment 1

[0137] 1 mL of an aqueous solution containing 10 mM salicylic acid (pH: 3.0) and 3000 ppm of dodecyltrimethylammonium bromide (DTAB) was placed in a thermostat at 35°C. Here, the various pigments shown in Figure 2 and Figure 3 (NK-1840 2ppm, NK-2929 6ppm, NK-3375 6ppm, NK-3662 6ppm, NK-5056 6ppm, NK-3620 6ppm, NK-9001 2ppm, NK-9002 2ppm, NK-9003 2ppm, NK-4249 2ppm and NK-3606 2ppm) were added to the above concentrations and dissolved respectively to obtain reagents for sample analysis.

[0138] Mix 1 mL of the obtained reagent with 20 μl of blood sample (Baso sample 1 or 2 or NRBC sample 1 or 2) thoroughly, react at 35°C for 20 seconds, take the sample out of the thermostat, and send it to a flow cytometer with a 633nm excitation light source detection part. The cells in the sample are stimulated to emit light and emit scattered light and fluorescence, and the scattered light and fluorescence signals emitted by the cells are detected. The resulting signal is analyzed to dete...

Embodiment 2

[0153] Except using the BN sample to replace the sample used in Example 1, and using reagents containing NK-2929, NK-3375, NK-3662 and NK-5056 as pigments, the others are the same as in Example 1, for basophils and Nucleated red blood cells were measured, and the ratio of basophils and nucleated red blood cells was calculated. The dye concentration in the reagent for sample analysis was adjusted to 6 ppm. These ratios are shown in Table 3. The scatter diagram drawn in this embodiment with the two axes of forward scattered light intensity and fluorescence intensity is shown in FIG. 4 .

[0154] [table 3]

[0155]

[0156]From the results in Fig. 4, it can be confirmed that basophils in the sample can be clearly distinguished from other components, and nucleated erythrocytes can be clearly separated from other components in a single measurement using the reagent for sample analysis of the present invention. It can be seen from Table 3 that the ratio calculated in Example 2...

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PUM

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Abstract

A reagent for measuring basophils and / or nucleated red blood cells in a sample, which comprises at least one fluorescent dye defined in the formula (I) or (II) is disclosed. Also disclosed are a kit and method for measuring basophils and / or nucleated red blood cells.

Description

Technical field: [0001] The present invention relates to a sample analysis reagent, a sample analysis kit, and a sample analysis method for analyzing blood cells in a sample collected from a living body. Background technique: [0002] In the field of clinical examination, analysis of blood cell components in a sample is very useful for diagnosing various diseases of the subject's circulatory organs and the like. Depending on the disease, the number of specific blood cells may increase or decrease, and blood cells that normally do not exist may appear in peripheral blood. [0003] In recent years, various automatic blood cell counting devices using flow cytometry have appeared on the market. People use the device to classify and count blood cells in general examination rooms. Using these automatic blood cell counting devices, blood cells in a sample can be automatically classified and counted. [0004] To classify and count white blood cells, red blood cells in a blood sam...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/14G01N15/12G01N21/64G01N33/48
CPCG01N33/5094
Inventor 水上利洋竹下浩生成川隆也
Owner SYSMEX CORP
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