Separation method for Leydig cell and use thereof

Abstract

The invention discloses a method for obtaining Leydig cells and purposes of the method. The method disclosed by the invention has the advantages of quickness, simpleness and convenience, and capability of obtaining a large quantity of Leydig cells with high purity and good functions, and is used for establishing seed cells of tissue engineered androgen-secreting tissue and other purposes.

Description

Leydig cell separation method and use thereof technical field The invention relates to seed cells in tissue engineering and cell transplantation, in particular to a method for separating interstitial cells of the testis and uses thereof. Background technique Androgen deficiency is common in many related clinical departments such as andrology, urology, and plastic surgery. In recent years, with the accelerated pace of life, increased work pressure, obesity and population aging, the number of patients with androgen deficiency has increased year by year, and has become a refractory disease that seriously threatens men's health and quality of life worldwide. disease. At present, there is no long-term stable effective measure for the treatment of androgen deficiency clinically. The more commonly used method is exogenous testosterone supplementation therapy, but testosterone supplementation therapy requires long-term application, and cannot meet the physiological requirements ...

AI Technical Summary

Problems solved by technology

At present, the classic methods to obtain high-purity Leydig cells are cell elutriation and Percoll density gradient centrifugation. The most prominent disadvantages are complicated separation techniques, many operating steps, and a long time.

After these two separation methods, the cells are very damaged, the acquisition rate is low, the survival rate is low, it is difficult to expand, and the long-term testosterone production function cannot be maintained in vitro, and it is difficult to meet the requirements of cell therapy and tissue engineering tissue construction. and the dual requirements of the function

Moreover, during these operations, a large amount of Leydig stem cells and precursor cells will be lost, resulting in the inability to maintain the number and function of Leydig cells for a long time

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