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Method for making rat have diabetic cardiac disease pathological characters

A technology for diabetes and heart disease, applied in medical science, surgery, diagnosis, etc., can solve the problem of lack of effective drugs for diabetic heart disease, and achieve the effects of easy promotion, small difference in clinical experiments, and easy operation

Inactive Publication Date: 2008-06-11
HARBIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is still a lack of effective drugs that can be used to prevent and treat diabetic heart disease. It is very important to verify the efficacy of drugs in the process of drug development. At present, there is no animal model that is economical, operable, and easy to promote. drug efficacy verification

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  • Method for making rat have diabetic cardiac disease pathological characters

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Effect test

specific Embodiment approach 1

[0007] Specific embodiment one: the method for making rats have the pathological characteristics of diabetic heart disease in this embodiment is carried out as follows: 1. Preparation of fat emulsion: 1 to 3 parts of cholesterol, 18 to 22 parts of lard, methyl 1-3 parts of thiouracil, 8-12 parts of sucrose and 5-8 parts of glucose are dissolved in 80-100 parts of soil temperature 80 at 30-45°C; Rats were gavaged for 10-15 days, with a daily dose of 8-10 mL / kg, and then intraperitoneal injection of streptozotocin by two-step administration method, with a daily dose of 35-50 mg / kg for each injection; three , Screening of rats: test the fasting blood glucose of the rats after 72 hours of streptozotocin injection, and select the rats whose fasting blood glucose value is greater than or equal to 16.7mmol / L as the type 2 diabetes model rats. The fat emulsion prepared in step 1 was gavaged for 30 days, and the daily gavage dose was 8-10 mL / kg; thus, rats with pathological characteris...

specific Embodiment approach 2

[0008] Specific embodiment two: the difference between this implementation method and specific embodiment one is: in step one, 2 parts of cholesterol, 20 parts of lard, 2 parts of methylthiouracil, 10 parts of sucrose and 6 parts of glucose are prepared at 35 ° C. Under the condition of soluble in 90 parts of soil temperature 80. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0009] Embodiment 3: The difference between this embodiment method and Embodiment 1 is that in Step 2, the fat emulsion in Step 1 is used to gavage rats for 11-14 days, and the daily gavage dose is 8.5-9.5 mL / kg. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention discloses a method which enables a rat to have pathological features of diabetic cardiopathy, and relates to a method which enables the rat to have pathological features of cardiopathy. The invention solves the problem of few reports on verification method for the drug effect for curing diabetic cardiopathy. The method which enables a rat to have pathological features of diabetic cardiopathy comprises steps as follows: preparing fat emulsion, carrying through intragastric administration and choosing rat, and then the rat with pathological features of diabetic cardiopathy is obtained. The invention has small difference between pathology and human's morbidity condition, is convenient to deeply analyze the pathological features of diabetic cardiopathy and drug effect.

Description

technical field [0001] The invention relates to a method for making rats have pathological characteristics of heart disease. Background technique [0002] The heart disease associated with diabetes includes diabetic cardiomyopathy caused by microvascular disease, arrhythmia and cardiac insufficiency caused by autonomic dysfunction. Since the invention and application of insulin and the use of antibacterial drugs, the incidence and mortality of ketone poisoning and microbial infection caused by diabetes have been greatly reduced, while the incidence and mortality of heart disease caused by diabetes have been significantly prominent and increased, and become the current leading cause of death in diabetic patients. At present, there is still a lack of effective drugs that can be used to prevent and treat diabetic heart disease. It is very important to verify the efficacy of drugs in the process of drug development. At present, there is no animal model that is economical, opera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B19/00A61B90/00
Inventor 艾静杨宝峰王宁闫新欣刘兰茹
Owner HARBIN MEDICAL UNIVERSITY