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Method for preparing total alkaloid

A technology for total alkaloids and plant extracts, applied in the field of preparation of total alkaloids, can solve the problems of low yield, difficult to improve the purity, no preparation method of total alkaloids, etc., and achieve the effect of increasing the content of alkaloids

Active Publication Date: 2008-08-20
JILIN SIHUAN PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] It comes down to one point, there is no widely applicable method for the preparation of general total alkaloids
Moreover, although the current method can make the content of total alkaloids higher than 50%, the yield is low and the purity is also difficult to improve

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation of embodiment 1- Motherwort total alkaloids

[0033] Take 300g of motherwort, add water and decoct three times, the first time for 2 hours, the second time for 1.5 hours, the third time for 1 hour, filter, combine the filtrate, concentrate to about 250ml, cool, add the same amount of ethanol, stir well, let stand Set aside for 24 hours, filtered, the filtrate was decompressed to recover ethanol and concentrated to a thick paste, diluted with water to 500ml, refrigerated for 24 hours, filtered, the filtrate was added to Nankai D151 weak acid cation exchange resin column, and eluted with water to obtain the Fraction A, then eluted with 4-5% ammonia water to obtain component B. Adjust the pH value of component A to 3 and add it to D151 strong acid cation exchange resin column to elute component C. Combine components B and C and purify them with neutral alumina column, and then evaporate the solvent to obtain the total organism of motherwort alkali.

Embodiment 2

[0034] Embodiment 2--The preparation of motherwort total alkaloids

[0035] Take 300g of motherwort, add water and decoct three times, the first time for 2 hours, the second time for 1.5 hours, the third time for 1 hour, filter, combine the filtrate, concentrate to about 250ml, cool, add the same amount of ethanol, stir well, let stand Set aside for 24 hours, filter, recover ethanol from the filtrate under reduced pressure and concentrate to a thick paste, dilute to 500ml with water, refrigerate for 24 hours, filter, add the filtrate to a GDX104 macroporous resin column, and elute with water to obtain component A , and then eluted with 50% ethanol to obtain component B. Adjust the pH value of component A to 1 and add it to the D018 strong acid cation exchange resin column to elute component C, combine components B and C with a neutral alumina column for purification, and then evaporate the solvent to obtain the total organism of motherwort alkali.

Embodiment 3

[0036] The preparation of embodiment 3- Motherwort total alkaloids

[0037] Take 300g of motherwort, add water and decoct three times, the first time for 2 hours, the second time for 1.5 hours, the third time for 1 hour, filter, combine the filtrate, concentrate to about 250ml, cool, add the same amount of ethanol, stir well, let stand Set aside for 24 hours, filtered, the filtrate was decompressed to recover ethanol and concentrated to a thick paste, diluted with water to 500ml, refrigerated for 24 hours, filtered, the filtrate was added to Nankai D151 weak acid cation exchange resin column, and eluted with water to obtain the Fraction A, then eluted with 4-5% ammonia water to obtain component B. Adjust the pH value of component A to 2.5 and add it to Nankai 001×1 strong acid cation exchange resin column to elute to obtain component C. Combine components B and C and purify them with neutral alumina column and evaporate the solvent. Obtain the total alkaloids of motherwort. ...

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PUM

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Abstract

The invention discloses a preparation method of a total alkaloid, which is characterized in that plant extracts are purified using weak acidic cation exchange resin, strong acidic cation exchange resin and neutral alumina to obtain the total alkaloids. According to the basic experiment, the general effective preparation method of total alkaloids has an advantage of effectively increasing the content of the total alkaloids.

Description

technical field [0001] The invention belongs to the technical field of separation and purification of alkaloids, and in particular relates to a preparation method of total alkaloids. Background technique [0002] Alkaloids generally refer to nitrogen-containing organic compounds in plants (except proteins, peptides, and amino acids). Alkaloids are the earliest natural products studied by humans. Most alkaloids have strong activity. [0003] Alkaloids are the most complex and largest class of compounds in natural products. There are more than 10,000 alkaloid monomer compounds that have been discovered, and these compounds are widely distributed in the genus Jew, Pinus, Picea, and Oilthorn. , Ephedra, Tritium and Liliaceae, Amaryllidaceae and Centipedeceae, Ranunculaceae, Magnoliaceae, Berberidaceae, Fangjiaceae, Aristolochiaceae, Papaveraceae, Tomatocladaceae, Rutaceae, Gentianae Branches, Apocynaceae, Marchanaceae, Rubiaceae, Solanaceae, Boraginaceae and Compositae. [0...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61K36/24A61K36/34A61K36/37A61K36/47A61K36/489A61K36/515A61K36/533A61K36/59A61K36/66A61K36/714A61K36/74A61K36/718A61K36/75A61K36/754A61K36/756A61K36/81A61K36/889A61K36/8966A61K36/8984A61K36/904C07G5/00
Inventor 魏海关
Owner JILIN SIHUAN PHARM CO LTD
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