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Injection for treating cancer and its preparation method

A technology for injections and tumors, applied in the field of medicine, can solve the problems of cumbersome preparation, difficult quality control, and inability to industrialize production, and achieve the effect of simple treatment method, simple preparation process, and good patient tolerance

Inactive Publication Date: 2010-11-17
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cytokines can enhance the immune function of one or more cells and have direct or indirect tumor killing effects, but cytokines non-specifically enhance the immune function of the body and do not have tumor cell-specific killing effects
At the same time, in order to play an obvious role in treating tumors clinically, cytokines require a large dose, resulting in increased toxic and side effects, so the application is limited.
[0011] (4) Immune cells with anti-tumor activity, such as lymphocyte-activated killer cells, cytokine-induced killer cells, tumor-infiltrating lymphocytes, etc. The advantage of this type of drug is that the effector cells induced in vitro avoid the tumor host Immunosuppressive, easy to activate and expand, activated killer cells can produce anti-tumor effects in vivo, its disadvantages are that it cannot be industrialized, the preparation is cumbersome, the quality control is difficult, the cost is high, and the effective tumor spectrum is not wide enough
There is no report on the application of erythrocyte membrane protein in tumor therapy

Method used

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  • Injection for treating cancer and its preparation method

Examples

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Comparison scheme
Effect test

example 1

[0057] 1, the preparation of injection of the present invention:

[0058] (1) Take type A blood from a healthy person, add 4 times the concentration of 0.01mol / L PBS, centrifuge at 3000r / min for 20 minutes, discard the supernatant, white blood cell layer and platelet layer, and wash with pre-cooled PBS equivalent to 4 times the volume of red blood cells 4 times, centrifuge at 5000r / min for 15min at 4°C each time to separate red blood cells and supernatant;

[0059] (2) Take the obtained red blood cells and add 45 times the volume of pre-cooled 0.01mol / L Tril-HCl, mix, and place at 4°C for 2 hours; then centrifuge at 9000r / min for 20min, and discard the supernatant;

[0060] (3) Repeat step (2) until there are no red blood cells visible to the naked eye to obtain a precipitate;

[0061] (4) Take the precipitate obtained in step (3) and dilute it with 0.01mol / L PBS, measure the content of erythrocyte membrane protein by Lowry's method, adjust the concentration to 20mg / ml, subpa...

example 2

[0063] (1) Take blood type B from a healthy person, add 5 times the concentration of PBS at 0.01mol / L, centrifuge at 3000r / min for 20min, discard the supernatant, white blood cell layer and platelet layer, and wash with pre-cooled PBS equivalent to 5 times the volume of red blood cells 3 times, centrifuge at 5000r / min for 15min at 4°C each time to separate red blood cells and supernatant;

[0064] (2) Take the obtained red blood cells and add 50 times the volume of pre-cooled 0.01mon / L Tril-HCl, mix, and place at 4°C for 2h; then centrifuge at 9000r / min for 20min, and discard the supernatant;

[0065] (3) Repeat step (2) until there are no red blood cells visible to the naked eye to obtain a precipitate;

[0066] (4) Take the precipitate obtained in step (3) and dilute it with 0.01mol / L PBS, measure the content of erythrocyte membrane protein by Lowry's method, adjust the concentration to 30mg / ml, subpackage, and store at -20°C.

example 3

[0068] (1) Take AB blood from a healthy person, add 3 times the concentration of 0.01mol / L PBS, centrifuge at 3000r / min for 20min, discard the supernatant, white blood cell layer and platelet layer, and wash with pre-cooled PBS equivalent to 3 times the volume of red blood cells 3 times, centrifuge at 5000r / min for 15min at 4°C each time to separate red blood cells and supernatant;

[0069] (2) Take the obtained red blood cells and add 40 times the volume of pre-cooled 0.01mol / L Tril-HCl, mix, and place at 4°C for 2 hours; then centrifuge at 9000r / min for 20min, and discard the supernatant;

[0070] (3) Repeat step (2) until there are no red blood cells visible to the naked eye to obtain a precipitate;

[0071] (4) Take the precipitate obtained in step (3) and dilute it with 0.01mol / L PBS, measure the content of erythrocyte membrane protein by Lowry's method, adjust the concentration to 10mg / ml, subpackage, and store at -20°C.

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Abstract

The invention relates to the use of human erythrocyte membrane during the preparation of the medicine for remedying tumors, also provides injection which embodies the use. The injection comprises human erythrocyte membrane albumen and buffer solution. The concentration of the human erythrocyte membrane albumen in the buffer solution is 10mg / ml-30mg / ml. The buffer solution is PBS or physiological saline solution. The injection of the invention can cure various solid tumors.

Description

technical field [0001] The invention relates to the field of medicine, in particular to medicines for treating tumors. Background technique [0002] The treatment methods for malignant tumors mainly include surgery, radiotherapy, chemotherapy, traditional Chinese medicine and immunotherapy. [0003] Surgical treatment is the most effective method to treat tumors, but it is difficult to prevent tumor recurrence and distant metastasis by relying on surgery alone, and it is difficult to achieve the goal of radical cure; although radiation therapy has a radical effect on some tumors, it still has certain limitations. Radiation therapy is a local treatment method, which can treat local lesions and cannot prevent distant metastasis of tumors; radiation therapy kills tumor cells by radiation, and some tumor cells that are not sensitive to radiation cannot be completely killed, resulting in Tumor residue and recurrence. Chemotherapy has a relatively high cure rate for some tumors,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61K9/08A61P35/00
Inventor 张积仁胡喜钢孙丽斌
Owner SOUTHERN MEDICAL UNIVERSITY
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