Use of Alstonia mairei extract in preparing anti-tumor medicament

A technology of anti-tumor drugs and extracts, which is applied in the field of application of cotton wool extracts in the preparation of anti-tumor drugs, can solve problems such as applications that have not been reported, and achieve the effect of low cost and simple method

Inactive Publication Date: 2011-08-10
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report on the application of sheep's horn cotton in the preparation of antitumor drugs

Method used

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  • Use of Alstonia mairei extract in preparing anti-tumor medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1, the preparation of cotton wool extract

[0018] Get croissant (Ningbo Dekang Biological Products Co., Ltd.) 200g, mix it with 95% ethanol (ethanol: water=95: 5, volume ratio) with the weight and number ratio of 1: 3 (croissant: 95% ethanol) , reflux extraction at 60°C for 3h, suction filtration, and collect the first reflux extract 1 and filter residue 1; mix all the filter residue 1 obtained with 95% ethanol, the amount of 95% ethanol is the same as the first time, at 60 Extract under the condition of ℃ for 3 hours, collect the second reflux extract 2 and filter residue 2, and combine the extract 2 and extract 1; mix all the filter residue 2 obtained with 95% ethanol, the amount of 95% ethanol is the same as that of the first Same, reflux extraction at 60°C for 3 hours, suction filtration, and collect the third reflux extract 3; combine extract 1, extract 2 and extract 3, concentrate the obtained extract under reduced pressure, and vacuum dry (remove etha...

Embodiment 2

[0019] Embodiment 2, the application of cotton wool extract in inhibiting the growth of tumor cells

[0020] The logarithmically grown tumor cells (human liver cancer cell HepG-2, human liver cancer cell BEL-7402, human colon cancer cell HCT-8, human lung cancer cell A-549) were digested with 0.25% trypsin, and then treated with 10% (volume percentage content) DMEM complete medium of fetal bovine serum suspends cells, makes cell suspension, and dilutes each tumor cell to 1.0×10 respectively 5 cells / mL, and then inoculated into 96-well culture plate, 200uL per well, each tumor cell was inoculated into 6 wells, and placed at 37°C, 5% CO 2 Cultivate in an incubator for 24 hours; then pour out the culture solution in each well.

[0021] The croissant extract prepared in Example 1 was dissolved in DMSO, then diluted with DMEM complete medium to obtain a solution of the croissant extract, and the final concentration of the croissant extract was 50ug / mL, wherein the concentration of...

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Abstract

The present invention discloses a new usage of alstonia extract. The new usage is the application of alstonia extract in preparing anti-tumor drugs. The extract is prepared according to the following method: taking alstonia as the raw material and extracting by water and / or organic solvent. The alstonia extract provided by the present invention plays an important role in preparing anti-tumor drugs, especially the drugs for resisting liver cancer, lung cancer and colon cancer.

Description

technical field [0001] The invention relates to the application of cotton wool extract in the preparation of antitumor drugs. Background technique [0002] Liver cancer is one of the common malignant tumors in our country, and it is the second cancer "killer" in our country, which is common in middle-aged men. Because of its high degree of malignancy and rapid disease progression, patients generally have no discomfort in the early stage, but once symptoms appear and go to the doctor, it is already in the middle and late stages. Therefore, the treatment of liver cancer is very difficult, and the average patient's survival time after onset is only 6 months, which is called "the king of cancer". According to the survey results, the top three new cancers in men are lung cancer, colon cancer, and nasopharyngeal cancer, and breast cancer, colon cancer, and lung cancer in women, which fully demonstrates the harm of lung cancer and colon cancer in the new cancer group sex. [000...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/24A61P35/00
Inventor 唐亚林谭莉戴玮刘岩向俊锋
Owner INST OF CHEM CHINESE ACAD OF SCI
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