Preparation and use of giant panda Ascaris schroederi antigen
A technology of Ascaris shizii and giant panda, which is applied in the field of preparation of Ascaris shizii from giant pandas, and can solve the problems of less research on immunodiagnostic technology of ascaris
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[0028] Embodiments are given and further descriptions are given in conjunction with the accompanying drawings. Firstly, collect roundworm eggs by giant panda feces precipitation method, put them in a culture dish containing 2% formalin in normal saline, cover them, and culture them in an incubator at 25-28°C, and a large amount of infectivity can be obtained in 3-4 weeks. Eggs, artificially infect white rabbits with giant panda roundworm infective eggs, and each rabbit was given 3,500 eggs, repeated every other week. Before each immunization, blood was collected from the ear vein of the experimental animal, and after four times of immunization, the titer was determined by agar expansion method. When the titer of the serum reached 1:8, blood was collected from the heart, and the separated serum was collected, divided into small tubes, and stored at -20°C for future use. The rabbit anti-Panda Ascaris positive serum was obtained for the next test. Take the collected giant panda ...
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