Rapid identification method of Chinese caterpillar fungus fertile bacterial strain

Abstract

The invention relates to a fast appraisal method of Cordyceps militaris fertile strains, which belongs to the edible fungus cultivation field. Single colonies which are sprouted by ascospore are selected, 100 single colonies are selected each time to be put under the constant temperature of 20 DEG C for culture for 16 days, with indoor natural lighting and the air humidity of 70 percent; strong strains with above 20 grass teeth, which grow uniformly, and have faint yellow colonies, golden yellow grass teeth, even growing trend, and no abnormalities are picked up, examined and confirmed by a microscope to obtain the fertile strain. The fertile strains are propagated and cultured for one time, the propagated strains are reproduced for 5 to 8 days by using a liquid media, and a mycelium pellet formed in the liquid becomes the strains for production. The method of the invention has short appraisal time and high accuracy. The time from the colony selection to the appraisal result only lasts for 20 days, more production cycles suitable for the two generation can be won for the Cordyceps militaris production, thereby further reducing the production cost, increasing the production speed, and improving the quality and the output of the Cordyceps militaris.

Description

1. Technical field [0001] The invention relates to a rapid identification method for fertile strains of Cordyceps militaris, belonging to the field of edible fungus cultivation. Including the new technology of breeding, screening and identification of Cordyceps militaris, this identification method can remove non-fruiting strains and obtain the first generation strains that grow grass completely. This technology is novel, practical and creative. 2. Background technology [0002] The edible fungi industry has been included in my country as one of the six major industries of grain, cotton, oil, fruit, tea, and edible fungi. At present, the annual production capacity is more than 16 million tons, and the export volume is 8 million tons. my country has become a major producer of edible fungi in the world. Medicinal fungi (or health-care fungi) are a newly developed edible fungus industry in my country in the past ten years. The market demand is large, and the cultivation volume...

AI Technical Summary

Problems solved by technology

[0006] The present invention aims at the problems of unstable species, easy degeneration of sterility, deformed fruiting body and decreased yield in the existing Cordyceps militaris cultivation and strain propagation technology, and provides a rapid screening of a fertile strain of Cordyceps militaris, The methods used for identification and production can solve the problems of strain sterility and deformed mushrooms that are prone to occur in production, and provide high-yield and high-efficiency technical support for the industrial cultivation of Cordyceps militaris in my country