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Preprocessing method of Orthoptera insect integer serial section

An Orthoptera pretreatment technology, applied in the preservation, application, and animal husbandry of human or animal bodies, to achieve high repeatability, good effect, and optimize the time of wax penetration

Inactive Publication Date: 2009-03-25
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the overall serial sectioning technique for Orthoptera insects, especially locusts, in my country.

Method used

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  • Preprocessing method of Orthoptera insect integer serial section
  • Preprocessing method of Orthoptera insect integer serial section
  • Preprocessing method of Orthoptera insect integer serial section

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1. The pretreatment method for the whole continuous slice of the third-instar locust fly

[0027] 1) Pre-treatment of materials: The third-instar locust fly of East Asian migratory locust is slightly frozen, fixed with 30 times Bouin’s fixative for 1 hour, the feet are removed, and a number of holes are pierced in the body of the locust with a dissecting needle.

[0028] 2) Material fixation: The locusts are placed in 50 times Bouin’s fixative solution, and vacuum is carried out. After fixation for 24 hours, they are washed three times with 70% alcohol for 10 minutes each.

[0029] 3) Dehydration treatment: dehydration with 70%, 80%, 90%, 95%, 100%, 100% gradient alcohol, each gradient is one hour, each gradient is subjected to negative pressure pumping, the final state of negative pressure pumping The pressure is -0.09MPa.

[0030] 4) Transparency treatment: pass through 50% alcohol + 50% xylene, xylene, xylene in sequence, 1 to 2 hours for each level, and stop ...

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Abstract

The invention discloses an entire orthoptera insect serial section pretreatment method, comprising the following steps: 1, freezing the orthoptera insect, fixing the insect in Bouin's fixative, removing feet, puncturing, and then placing the insect in the Bouin's fixative, and fixing the insect through negative pressure pumping; 2, carrying out gradient dehydration treatment under the conditions of negative pressure pumping; 3, carrying out transparency treatment under the conditions of negative pressure pumping; and 4, carrying out paraffin infiltration treatment with paraffin. The pretreatment method carries out negative pressure pumping in the fixation, dehydration and paraffin invasion processes, and can improve the treatment efficiency and enables the processes to be more thorough; the method optimizes the paraffin invasion time and enables the paraffin to be invaded in to each part of the orthoptera insect body, and also eliminates the complicated step of specially softening the tegument of the insect. The method can be used to obtain complete serial sections and has good effect and high repeatability, which is suitable for entire orthoptera insect serial sections.

Description

Technical field [0001] The invention relates to a microscopic tissue slicing technique, in particular to a pretreatment method suitable for continuous slices of orthopteran insects. Background technique [0002] For the study of orthopteran insects, especially locust tissue slices, common slicing techniques are basically taking materials from specific parts and performing slices. However, due to the obstruction of the body wall of locusts, common slicing techniques are fixed, dehydrated, transparent, and wax invaded. The links are not thorough, and it is not easy to grasp the time. It is easy to cause the slices to break when slicing, and it is impossible to perform a good overall continuous slice of the locust. The continuous sectioning of the locust is of great significance. It can accurately understand the overall state of the various organs of the locust under specific time and conditions. It can not only study the overall development of the various organs of the locust in di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00
Inventor 张龙尹学伟
Owner CHINA AGRI UNIV
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