Monoclonal antibody resisting anaplasma marginale MSP5 protein and application thereof

Abstract

The invention discloses a monoclonal antibody resisting anaplasma marginale MSP5 protein and an application thereof. The invention adopts the purified anaplasma marginale MSP5 protein expressed by escherichia coli as the immunogen to immune mice; a hybridoma cell line which can steadily secrete monoclonal antibody resisting anaplasma marginale MSP5 protein is obtained by cell fusion and screening; and the micromechanism collection number is CGMCC No.3205. The monoclonal antibody secreted by the hybridoma cell line can react with the anaplasma marginale MSP5, simultaneously can carry out idiosyncratic reaction with the cattle anaplasma marginale and the sheep anaplasma marginale, and can not react with mycoplasmas, neospora canimum, eperyt hrozoon and the like. The monoclonal antibody in the invention can be used for differentiating anaplasma marginale and other related pathogenies and provides material basis for the building of a rapid, convenient and accurate diagnostic method and for the immune mechanism research, immune function detection, detection method establishment and the like.

Description

technical field [0001] The invention relates to a monoclonal antibody, in particular to a monoclonal antibody against marginal aplasma MSP5 protein, and also relates to a hybridoma cell strain secreting the anti-MSP5 protein monoclonal antibody, which belongs to the field of cell engineering. Background technique [0002] Anaplasma disease, formerly known as anaplasmosis, is caused by Rickettsiales (Rickettsiales), Anaplasmataceae (Anaplasmataceae), Anaplasma marginale (Anaplasma marginale), central aplasma (A. centrale) and sheep aplasma (A.bovis) and other species parasitizing in red blood cells, mainly spread by blood-sucking arthropods (ticks, gadflies, stable flies, mosquitoes, etc.), and are transmitted by ticks. Parasitic Protozoa is the most widely distributed in the disease (Ristic M. Bovine anaplasmosis. Parasitic Protozoa, edited by Kreier J P, 1977, (IV): 235-249). Marginal and central anaplasts were first discovered in North Africa in 1910 and 1911. Since then...

AI Technical Summary

Problems solved by technology

At present, due to the fact that the domestic research on anplasma borderline has just been carried out, there is no report on its monoclonal antibody, which limits the application of monoclonal antibodies in the clinical diagnosis of anplasma borderline to a large extent. Therefore, The development of an ELISA diagnostic kit for detection of borderline aplasma disease is of great significance for the disease's epidemic monitoring, epidemiological investigation, and improvement of immunization strategies (Zhou Jie, Jiang Mo, Zhang Hongying, etc. Recombinant nucleocapsid of canine distemper virus Preparation and Identification of Protein Monoclonal Antibody [J], Chinese Journal of Preventive Veterinary Medicine, 2007, 29(7): 528-532)

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