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Methods of hydrolyzing soyabean protein to produce exorphins

A technology of hydrolyzing soybean protein and trypsin, which is applied in the direction of fermentation, etc., can solve the problems of hydrolyzing soybean protein ignoring the physiological function and safety hazards of opioid peptides, and achieve the effects of strengthening physiological functions, regulating digestive tract movement, and improving secretion

Inactive Publication Date: 2012-02-29
HARBIN UNIV OF COMMERCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problem of ignoring the physiological function and potential safety hazards of opioid peptides by using the existing method of hydrolyzing soybean protein, and provides a method for hydrolyzing soybean protein to produce exorphins

Method used

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  • Methods of hydrolyzing soyabean protein to produce exorphins
  • Methods of hydrolyzing soyabean protein to produce exorphins
  • Methods of hydrolyzing soyabean protein to produce exorphins

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specific Embodiment approach 1

[0017] Specific embodiment one: the method for producing exorphins by hydrolyzing soybean protein in this embodiment is carried out as follows: using trypsin / alkaline enzyme, the pH value is 8.0, the temperature is 55 ° C, and the enzyme to substrate concentration ratio is 1: 3 under the conditions of enzymatic hydrolysis for 5h.

[0018] In this embodiment, the optimal dosage of trypsin is 8000U / g, and the optimal dosage of alkaline enzyme is 6000U / g.

[0019] The method of hydrolyzing soybean protein to produce exorphin in this embodiment The exorphin produced by hydrolyzing soybean protein is an antagonist, and the average peptide chain length is 7.85.

[0020] The liquid chromatogram of the hydrolyzate of the present embodiment is as figure 1 shown, from figure 1 It can be seen that the hydrolyzed soybean protein of this embodiment produces exorphins as antagonists.

specific Embodiment approach 2

[0021] Specific embodiment two: The method for producing exorphins by hydrolyzing soybean protein in this embodiment is carried out as follows: using trypsin / neutral enzyme, the pH value is 7.5, the temperature is 55 ° C, and the enzyme and substrate concentration ratio is 2: 1 under the condition of enzymatic hydrolysis for 5h.

[0022] In this embodiment, the optimal dosage of trypsin is 8000U / g, and the optimal dosage of neutral enzyme is 6000U / g.

[0023] The method of hydrolyzing soybean protein to produce exorphin in this embodiment hydrolyzes soybean protein to produce exorphin as an agonist, the target exorphin percentage is 0.007275%, and the average peptide chain length is 7.85.

[0024] The liquid chromatogram of the hydrolyzate of the present embodiment is as figure 2 shown, from figure 2 It can be seen that the hydrolyzed soybean protein of this embodiment produces exorphins as agonists.

specific Embodiment approach 3

[0025] Specific embodiment three: the method for producing exorphins by hydrolyzing soybean protein in this embodiment is carried out as follows: using pancreatic alkaline enzyme / neutras (Alcalase / Neutras), at a pH value of 7.5, a temperature of 55 ° C, enzyme and Enzymolysis was carried out for 5 hours under the condition that the substrate concentration ratio was 3:1.

[0026] In this embodiment, the optimal dosage of alkaline enzyme is 6000U / g, and the optimal dosage of neutral enzyme is 6000U / g.

[0027] The method of hydrolyzing soybean protein to produce exorphin in this embodiment hydrolyzes soybean protein to produce exorphin as an agonist, the target exorphin percentage is 0.01228%, and the average peptide chain length is 6.68.

[0028] The liquid chromatogram of the hydrolyzate of the present embodiment is as image 3 shown, from image 3 It can be seen that the hydrolyzed soybean protein of this embodiment produces exorphins as agonists.

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Abstract

The invention relates to methods of hydrolyzing soyabean protein to produce exorphins, belonging to the method of hydrolyzing the soyabean protein to produce special peptide and aiming at solving the problem that the prior method of the soyabean protein to produce the opioid peptide ignores the physiological function and the safety danger of the opioid peptide. The four methods of hydrolyzing thesoyabean protein to producing the exorphins are implemented in corresponding conditions by using parenzyme / alkaline enzyme, parenzyme / neutral enzyme, pancreatic alkaline enzyme / neutral enzyme and acid proteolytic enzyme / pepsinum. The four methods of synchronously hydrolyzing the soyabean protein to produce exorphins ensure the condition of producing the exorphins and the amount of the produced exorphins and lays a foundation for the safety of the post processing of the soyabean protein and the development of the soybean peptide food.

Description

technical field [0001] The present invention relates to methods for hydrolyzing proteins to produce specific peptides. Background technique [0002] In recent years, research on bioactive peptides from food sources has developed rapidly. Because soybeans are rich in resources and are relatively cheap and easy-to-obtain protein raw materials, the research, development and production of soybean peptides are extremely active. With the continuous deepening of the development of soybean deep-processing products, soybean peptides as a new soybean product have received widespread attention, and functional foods based on soybean peptides have also been continuously developed, becoming a hot spot in the field of food research. But in general, the research and development of soybean peptides are more focused on its functional properties and applications, while the research on the food safety of soybean peptides is less, almost blank. [0003] Exorphins have a wide range of sources, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
Inventor 石彦国孙冰玉胡春林
Owner HARBIN UNIV OF COMMERCE
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