Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Methods of hydrolyzing soyabean protein to produce exorphins

A technology for hydrolyzing soybean protein and pepsin, applied in the direction of fermentation, can solve the problem of hydrolyzed soybean protein neglecting the physiological function of opioid peptides and hidden safety hazards, and achieve the effect of strong physiological function, glucagon secretion promotion and secretion improvement.

Inactive Publication Date: 2010-04-07
HARBIN UNIV OF COMMERCE
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problem of ignoring the physiological function and potential safety hazards of opioid peptides by using the existing method of hydrolyzing soybean protein, and provides a method for hydrolyzing soybean protein to produce exorphins

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methods of hydrolyzing soyabean protein to produce exorphins
  • Methods of hydrolyzing soyabean protein to produce exorphins
  • Methods of hydrolyzing soyabean protein to produce exorphins

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0017] Specific embodiment one: the method for producing exorphins by hydrolyzing soybean protein in this embodiment is carried out as follows: using trypsin / alkaline enzyme, the pH value is 8.0, the temperature is 55 ° C, and the enzyme to substrate concentration ratio is 1: 3 under the conditions of enzymatic hydrolysis for 5h.

[0018] In this embodiment, the optimal dosage of trypsin is 8000U / g, and the optimal dosage of alkaline enzyme is 6000U / g.

[0019] The method of hydrolyzing soybean protein to produce exorphin in this embodiment The exorphin produced by hydrolyzing soybean protein is an antagonist, and the average peptide chain length is 7.85.

[0020] The liquid chromatogram of the hydrolyzate of the present embodiment is as figure 1 shown, from figure 1 It can be seen that the hydrolyzed soybean protein of this embodiment produces exorphins as antagonists.

specific Embodiment approach 2

[0021] Specific embodiment two: The method for producing exorphins by hydrolyzing soybean protein in this embodiment is carried out as follows: using trypsin / neutral enzyme, the pH value is 7.5, the temperature is 55 ° C, and the enzyme and substrate concentration ratio is 2: 1 under the condition of enzymatic hydrolysis for 5h.

[0022] In this embodiment, the optimal dosage of trypsin is 8000U / g, and the optimal dosage of neutral enzyme is 6000U / g.

[0023] The method of hydrolyzing soybean protein to produce exorphin in this embodiment hydrolyzes soybean protein to produce exorphin as an agonist, the target exorphin percentage is 0.007275%, and the average peptide chain length is 7.85.

[0024] The liquid chromatogram of the hydrolyzate of the present embodiment is as figure 2 shown, from figure 2 It can be seen that the hydrolyzed soybean protein of this embodiment produces exorphins as agonists.

specific Embodiment approach 3

[0025] Specific embodiment three: the method for producing exorphins by hydrolyzing soybean protein in this embodiment is carried out as follows: using pancreatic alkaline enzyme / neutras (Alcalase / Neutras), at a pH value of 7.5, a temperature of 55 ° C, enzyme and Enzymolysis was carried out for 5 hours under the condition that the substrate concentration ratio was 3:1.

[0026] In this embodiment, the optimal dosage of alkaline enzyme is 6000U / g, and the optimal dosage of neutral enzyme is 6000U / g.

[0027] The method of hydrolyzing soybean protein to produce exorphin in this embodiment hydrolyzes soybean protein to produce exorphin as an agonist, the target exorphin percentage is 0.01228%, and the average peptide chain length is 6.68.

[0028] The liquid chromatogram of the hydrolyzate of the present embodiment is as image 3 shown, from image 3 It can be seen that the hydrolyzed soybean protein of this embodiment produces exorphins as agonists.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to methods of hydrolyzing soyabean protein to produce exorphins, belonging to the method of hydrolyzing the soyabean protein to produce special peptide and aiming at solving the problem that the prior method of the soyabean protein to produce the opioid peptide ignores the physiological function and the safety danger of the opioid peptide. The four methods of hydrolyzing the soyabean protein to producing the exorphins are implemented in corresponding conditions by using parenzyme / alkaline enzyme, parenzyme / neutral enzyme, pancreatic alkaline enzyme / neutral enzyme and acid proteolytic enzyme / pepsinum. The four methods of synchronously hydrolyzing the soyabean protein to produce exorphins ensure the condition of producing the exorphins and the amount of the produced exorphins and lays a foundation for the safety of the post processing of the soyabean protein and the development of the soybean peptide food.

Description

technical field [0001] The present invention relates to methods for hydrolyzing proteins to produce specific peptides. Background technique [0002] In recent years, research on bioactive peptides from food sources has developed rapidly. Because soybeans are rich in resources and are relatively cheap and easy-to-obtain protein raw materials, the research, development and production of soybean peptides are extremely active. With the continuous deepening of the development of soybean deep-processing products, soybean peptides as a new soybean product have received widespread attention, and functional foods based on soybean peptides have also been continuously developed, becoming a hot spot in the field of food research. But in general, the research and development of soybean peptides are more focused on its functional properties and applications, while the research on the food safety of soybean peptides is less, almost blank. [0003] Exorphins have a wide range of sources, a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 石彦国孙冰玉胡春林
Owner HARBIN UNIV OF COMMERCE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com