A kind of preparation method of high-purity 95 isomaltooligosaccharide

Abstract

The invention provides a preparation method of high-purity 95 isomaltose hypgather. The preparation method comprises the following steps: performing jet liquefying on starch or starch milk used as a raw material; performing saccharification transglycosidation through maltase (fungus alpha-amylase or beta-amylase) and alpha-galctosyl-hydroxylysyl glucosyl transferase to obtain 50 type coarse isomaltose hypgather liquor; decomposing non-trisaccharide (isomaltose, panose and isomaltotriose hereinafter referred to as 'trisaccharide') ingredients by use of efficient liquid saccharifying enzyme to obtain glucose; separating by use of a chromatographic separation purification technology to remove glucose; decolorizing, desalting and refining to obtain 95 isomaltose hypgather (the 'trisaccharide' content is not less than 95%). The high-purity 95 isomaltose hypgather has the advantages that the effective ingredients 'trisaccharide' are high in purity reaching more than 95%, low in syrup viscosity and high in sweetness; the physiology functions are high; the bifidobacterium cultivation effect is three times higher than that of the common 90 type isomaltose hypgather.

Description

technical field

[0001] The invention belongs to the technical field of functional oligosaccharide preparation, and in particular relates to a preparation method of high-purity 95 isomaltooligosaccharide. Background technique

[0002] Isomaltooligosaccharide, also known as branched oligosaccharide, is a functional oligosaccharide made from starch through glycosylation and transglycoside conversion with biological enzymes. Isomaltooligosaccharide has good moisture retention and can inhibit the retrogradation and aging of starch and the precipitation of crystalline sugar in food; the "trisaccharide" in isomaltooligosaccharide (refers to isomaltose + panose + isomaltotriose, the same below) It has good proliferative activity of bifidobacteria, can inhibit the growth of harmful bacteria, can effectively prevent constipation, promote the absorption of minerals such as calcium and magnesium, and has low dental caries. It is widely used in food, beverages, and health products. It is...