Method for determining cross antigen region initiating human antigens in human enterovirus 71-type total protein

A human enterovirus, cross-immunity technology, applied in the field of biomedicine

Inactive Publication Date: 2010-04-28
INST OF LAB ANIMAL SCI CHINESE ACAD OF MEDICAL SCI
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Problems solved by technology

[0006] However, at the same time, comparative medical experiments in mice showed that the onset of neonatal mice was not accompanied by massive replication of the virus in the brain tissue, which posed new challenges to

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  • Method for determining cross antigen region initiating human antigens in human enterovirus 71-type total protein

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Embodiment Construction

[0019] The present invention will be described in further detail below in conjunction with specific embodiment, but it is not limited thereto:

[0020] Implementation step one

[0021] Segmentation and engineered expression of human enterovirus type 71 total protein

[0022] The template on which the segmentation relies is the human enterovirus 71 isolate from Fuyang, Anhui Province, China, with Genbank accession number EU703812. The genome CDS region of the strain is 6582bp in total, encoding 2193 amino acids. The size of the split polypeptide is controlled at about 100 amino acids (aa), the lower limit is 22aa, and the upper limit is 156aa. There are 4 Overlap of ~15 aa, as shown in Table 1.

[0023] Table 1 The segmentation method of human enterovirus type 71 total protein

[0024]

[0025] Add NdeI and SacI sites to both ends of the primers for amplifying the polypeptide coding region. The expression vector uses the modified pET28a(+) expression vector. After the vec...

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Abstract

The invention belongs to the field of biomedicine, and provides a method for determining a cross antigen region initiating human antigens in human enterovirus 71-type (EV71) total protein. In the method, the human enterovirus 71-type total protein is identified through segmental expression; and cross reaction between immunogenic and inductive antibodies and the human antigens in each segment is performed, and different segments of the human enterovirus 71-type total protein are divided into three classes: (1) strongly-crossed immunogens initiating the human antigens; (2) weakly-crossed immunogens initiating the human antigens; and (3) no crossed immunogens existing between the antibodies and the human antigens. Thus, the method plays a guidance role in developing and preparing human enterovirus 71-type vaccines, and hand-foot-and-mouth disease vaccines having no cross reaction or weak cross reaction with human bodies can be designed according to the method.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to a method for studying human enterovirus 71. Background technique [0002] Human enterovirus type 71 (EV71) was isolated and identified for the first time in 1969 and is one of the pathogens of HFMD. The virus mainly infects children under the age of 5, mainly causing hand, foot and mouth disease and herpetic angina. In a few cases, complications include meningitis, pulmonary edema, hemorrhage, etc., and severe cases can lead to death. Human enterovirus 71 has caused many disease outbreaks worldwide. Since the 1980s, hand, foot and mouth disease caused by human enterovirus 71 has been raging in the Asia-Pacific region, causing serious infant deaths. [0003] The disease was discovered in Shanghai in my country in 1981, and has been reported in various provinces and cities since then. In March 2009, 54,713 cases of hand, foot and mouth disease were reported through the direct network r...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/68
Inventor 秦川刘江宁张连峰李万波
Owner INST OF LAB ANIMAL SCI CHINESE ACAD OF MEDICAL SCI
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