Method for breeding Pietrain pigs
A technology of Pietrain and Pietrain, which is applied in animal husbandry and other directions, can solve the problems of homozygous HalNHalN Pietrain carcass traits such as reduced lean meat rate.
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Embodiment 1
[0027] Embodiment 1 establishes basic selection breeding population
[0028] The ear tissue samples of Pietrain pigs from the experimental pig farm of the Institute of Animal Husbandry and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, the Pietrain pigs from the breeding pig farm of Shanghai Dajiang Livestock and Poultry Co. [Tan Yongsong, Zhang Siqing, Zhang Zhongming, He Yuying, Liu Honglin, Wang Linyun. Application of PCR-RFLPs detection method in colonization of Pietrain pigs without halothane recessive gene. Shanghai Agricultural Journal, 2001, 17(3): 34-37] reported PCR-RFLPs method for detection of halothane gene in Pietrain pigs.
[0029] And recorded the age of 100kg, feed conversion rate, live backfat thickness, eye muscle area and other indicators of each Pietrain pig in the experimental pig farm of the Institute of Animal Husbandry and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, and calculated the comprehensive selection index S...
Embodiment 2
[0037] Example 2: Introducing Pietrain pig blood with the HalN gene to generate first generation pigs
[0038]The French Pietrain pig semen containing the N gene was imported from France, and the 10 Pietrain sows in the basic breeding population of Example 1 were artificially inseminated according to conventional methods in time to produce one generation, and the PCR-RFLPs method was used to detect one generation Pig Hal gene.
[0039] Results: After testing, a total of 127 piglets were produced, and 19 Pietrain heterozygous piglets with HalN gene were included in the breeding group.
Embodiment 3
[0040] Example 3 Breeding to expand the individual number of HalN gene Pietrain pigs and the emergence of HalNHalN genotype Pietrain pig individuals
[0041] The mating pattern of HalNHaln×HalNHaln was adopted to expand the number of Pietrain pigs containing HalN gene as much as possible, and it was also expected to obtain Pietrain pigs of HalNHalN genotype. At the same time, in order to make full use of the HalN gene of Pietrain boars, the mating pattern of HalNHaln×HalnHaln is also adopted to expand the number of Pietrain pigs containing HalN genes.
[0042] Pietrain boars and sows are cultivated by the combination of ancestry establishment and group subculture, and piglets are born and selected according to the above mating mode, and detailed archives are established for each Pietrain pig. Ear tissue samples were collected from piglets at birth, and the halothane gene and its genotype were detected by PCR-RFLPs; piglets were selected for the first time when they were 28 day...
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