Piezoelectric immuno-sensing method for detecting 2,4-dichlorphenoxyacetic acid in agricultural product

A dichlorophenoxyacetic acid and piezoelectric immunosensing technology, which is applied in the direction of material inspection products, measuring devices, biological testing, etc., can solve the problems of low sensitivity, complicated sample pretreatment, long measurement cycle, etc.

Inactive Publication Date: 2010-07-21
HUNAN AGRICULTURAL UNIV
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Problems solved by technology

However, these traditional methods have certain limitations, such as: complicated sample pretreatment, cumbersome and time-consuming operation, long measurement cycle, expensive equipment, low sensitivity, etc.

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  • Piezoelectric immuno-sensing method for detecting 2,4-dichlorphenoxyacetic acid in agricultural product

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Embodiment Construction

[0012] The present invention will be further described below in conjunction with the examples.

[0013] 1. Reagents and instruments

[0014] Cysteamine (Cys, ACROS company), BSA-2, 4-D conjugated antigen (BSA-2, 4-D) were synthesized and prepared by the Key Laboratory of Plant Hormones and Growth and Development of Hunan Province; glutaraldehyde was purchased from Fluka company Products; mouse anti-2,4-D antibody was entrusted to Beijing Boaosen Biotechnology Co., Ltd.; goat anti-mouse IgG antibody (G-Anti-MsIgG) and bovine serum albumin (BSA) were purchased from Beijing Dingguo Biotechnology Co., Ltd.; Chlorauric acid and sodium citrate were purchased from Shanghai Chemical Reagent Company; PBS solutions with different pH values ​​were prepared with 0.01M Na 2 HPO 4 and KH 2 PO 4 Preparation; Piranha reagent is H prepared in a volume ratio of 3:7 2 o 2 -H 2 SO 4 Mixed solution; other reagents are analytically pure; water used in the experiment is twice distilled water...

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Abstract

The invention relates to an piezoelectric immuno-sensing method for detecting 2,4-dichlorphenoxyacetic acid (2,4-D) content in agricultural products, mainly comprising the following steps: 1) coupling 2,4-dichlorphenoxyacetic acid with BSA; 2) preparing an electrode modification immunosensor; 3) preparing a Goat anti Mouse IgG antibody marked with nanogold; 4) preparing a standard curve; and 5) detecting a sample. The invention has the beneficial effect: during detection, the Goat anti Mouse IgG antibody marked with nanogold magnifies a signal to improve detection sensitivity so as to realize the high-sensitivity quantitative detection of 2,4-D. The invention is in linear relation when 2,4-D concentration is 13.3 ng.mL<-1>-666 ng.mL<-1> and has the lower limit of detection of 13 ng.mL<-1>. Meanwhile, the method has the advantages of simple method, reliable result, low cost and the like.

Description

technical field [0001] The invention relates to a biological detection method, in particular to a piezoelectric immunosensing method for detecting 2,4-dichlorophenoxyacetic acid in agricultural products. Background technique [0002] 2,4-D is a widely used plant growth regulator, and its determination methods include extraction-photometry, liquid chromatography, liquid chromatography-mass spectrometry, thin-layer chromatography, and derivative gas chromatography. However, these traditional methods have certain limitations, such as: complicated sample pretreatment, tedious and time-consuming operation, long measurement cycle, expensive equipment, and low sensitivity. Immunosensor is an automatic analysis and detection system formed by utilizing the high sensitivity, simplicity, and speed of electrochemical immunosensors, combined with a biorecognition system (antigen-antibody specific binding), which has the advantages of simple operation, rapidity, and high sensitivity. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/577G01N27/414
Inventor 肖浪涛王若仲蔺万煌刘清
Owner HUNAN AGRICULTURAL UNIV
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