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Electrochemistry immuno-sensing method for detecting 2,4-dichlorphenoxyacetic acid in agricultural product

A technology of dichlorophenoxyacetic acid and immunosensor, which is applied in measuring devices, scientific instruments, and material analysis through electromagnetic means, and can solve the problems of complex sample pretreatment, low sensitivity, and expensive equipment

Inactive Publication Date: 2010-07-07
HUNAN AGRICULTURAL UNIV
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Problems solved by technology

However, these traditional methods have certain limitations, such as: complicated sample pretreatment, cumbersome and time-consuming operation, long measurement cycle, expensive equipment, low sensitivity, etc.

Method used

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  • Electrochemistry immuno-sensing method for detecting 2,4-dichlorphenoxyacetic acid in agricultural product

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Embodiment Construction

[0012] The present invention will be further described below in conjunction with the examples.

[0013] 1. Reagents and instruments

[0014] 2,4-D, 1,6-hexanedithiol, chloroauric acid, BSA, trisodium citrate were purchased from Sigma, potassium ferricyanide, potassium ferrocyanide, potassium phosphorus chloride, EDC, NHS , 1,4-dioxane were purchased from Shanghai Chemical Reagent Company. All other reagents were of analytical grade, and the experimental water was twice distilled water.

[0015] CHI660C electrochemical workstation, gold electrode, Ag / Agcl saturated electrode, and platinum wire electrode were all purchased from Shanghai Chenhua Instrument Company, BRANSONIC200 ultrasonic cleaning instrument (Germany BRANSON ULTRASCHALL company), CS501-SP super digital display thermostat (Chongqing Sida Experimental Instrument Factory), MP230 acidity meter (Metter Toledo, Switzerland), AB204-S electronic balance (Metter Toledo, Switzerland), 2,4-D monoclonal antibody.

[0016]...

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Abstract

The invention relates to an electrochemistry immuno-sensing method for detecting 2,4-dichlorphenoxyacetic acid in agricultural products, mainly comprising the following steps: 1) coupling 2,4-dichlorphenoxyacetic acid with BSA; 2) preparing an electrode modification immunol sensor; 3) preparing a Goat anti Mouse IgG antibody with an HRP sign; 4) preparing a standard curve; and 5) detecting a sample. The invention has the beneficial effect that the Goat anti Mouse IgG antibody with an HRP sign is used for magnifying a signal and improving detection sensitivity so as to realize 2,4-D high-sensitivity quantitative determination. The method becomes liner relationship when 2,4-D density is within the range of 0.5-2,500 ng / mL and detection lower limit is 0.05 ng / ml, can better satisfy 2,4-D detection requirements in agricultural products, and has the advantages of simple method, reliable result, low cost and the like.

Description

technical field [0001] The invention relates to a biological detection method, in particular to an electrochemical immunosensing method for detecting 2,4-dichlorophenoxyacetic acid in agricultural products. Background technique [0002] 2,4-D is a widely used plant growth regulator, and its determination methods include extraction-photometry, liquid chromatography, liquid chromatography-mass spectrometry, thin-layer chromatography, and derivative gas chromatography. However, these traditional methods have certain limitations, such as: complicated sample pretreatment, tedious and time-consuming operation, long measurement cycle, expensive equipment, and low sensitivity. Immunosensor is an automatic analysis and detection system formed by utilizing the high sensitivity, simplicity, and speed of electrochemical immunosensors, combined with a biorecognition system (antigen-antibody specific binding), with simple, fast, cheap, streamlined, sensitive It has high advantages and ha...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N33/531
Inventor 肖浪涛李合松夏石头刘清
Owner HUNAN AGRICULTURAL UNIV
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