The invention discloses a method for simultaneous determination of xanthoxylin and capsaicin in food, comprising the following steps: 1), preparation of mixed standard working solution, 2), instrument configuration, 3), drawing standard curve, 4), sample preparation , 5), sample solution preparation, 6), constant volume, 7), chromatographic analysis, 8), result analysis. The invention provides a method for simultaneous determination of xanthoxylin and capsaicin in food, which has the characteristics of simplicity, quickness, good accuracy and high sensitivity, effectively solves the problem of numbness and spiciness monitoring of terminal products such as spicy gluten food, etc. practical application value.