Method for using petunia petal pigment as acid-base indicator
A technology of acid-base indicator and petunia, which is applied in the application field of chemical chromogen, can solve the problems of cumbersome extraction methods, low precision, and wide color range, and achieve rich color changes, easy and fast determination, and color development high precision effect
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Embodiment 1
[0013] (1) Take the petunia purple petals, cut them into pieces, grind them thoroughly with a mortar, add methanol solution containing 0.1% hydrochloric acid for leaching for 20 minutes according to the ratio of material to liquid (1:5 (w / v)), and take the purple supernatant stand-by;
[0014] (2) Use HCl, HAC, NH at a concentration of 1mol / L 3 ·H 2 O. Prepare 5 ml of NaOH solutions with a pH of 1 to 14, and add 10 drops to the holes of the white drip plate;
[0015] (3) Take the petunia purple petal pigment solution extracted in (1) with a dropper, add 2 drops respectively to the holes of the white drip plate containing different pH solutions, and observe the color of the solution. It was found that when the pH of the solution was 1-7, the color of the solution gradually changed from deep red to light red; when the pH was 8-10, the color of the solution changed from purple to blue; when the pH was 11-13, the color of the solution was grass green; At pH 14, the solution col...
Embodiment 2
[0018] (1) Take the petunia purple petals, cut them into pieces, grind them thoroughly with a mortar, add methanol solution containing 0.1% hydrochloric acid for leaching for 30 minutes according to the ratio of material to liquid 1:10 (w / v), and take the purple supernatant stand-by;
[0019] (2) Use HCl, HAC, NH at a concentration of 1mol / L 3 ·H 2 O. Prepare 5 ml of NaOH solutions with a pH of 1 to 14, and add 10 drops to the holes of the white drip plate;
[0020] (3) Take the petunia purple petal pigment solution extracted in (1) with a dropper, add 3 drops respectively to the holes of the white drip plate containing different pH solutions, and observe the color of the solution. It was found that when the pH of the solution was 1-7, the color of the solution gradually changed from deep red to light red; when the pH was 8-10, the color of the solution changed from purple to blue; when the pH was 11-13, the color of the solution was grass green; At pH 14, the solution colo...
Embodiment 3
[0023] (1) Take the petunia purple petals, cut them into pieces, grind them thoroughly with a mortar, add methanol solution containing 0.1% hydrochloric acid for leaching for 30 minutes according to the ratio of solid to liquid 1:5 (w / v), and take the purple supernatant stand-by;
[0024] (2) Use HCl, HAC, NH at a concentration of 1mol / L 3 ·H 2 O. Prepare 5 ml of NaOH solutions with a pH of 1 to 14, and add 10 drops to the holes of the white drip plate;
[0025] (3) Take the petunia purple petal pigment solution extracted in (1) with a dropper, add 2 drops respectively to the holes of the white drip plate containing different pH solutions, and observe the color of the solution. It was found that when the pH of the solution was 1-7, the color of the solution gradually changed from deep red to light red; when the pH was 8-10, the color of the solution changed from purple to blue; when the pH was 11-13, the color of the solution was grass green; At pH 14, the solution color wa...
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