Specific synthetic polypeptide for detecting novel H1N1 influenza viruses

A technology for synthesizing polypeptides and influenza viruses, applied in the field of synthetic polypeptides, can solve the problems of high cost, difficult to promote and apply at border ports, and long time for influenza virus isolation and identification, and achieves strong specificity, large market value and social benefits, and false negatives. The effect of low rate and background response

Inactive Publication Date: 2013-08-28
CHINESE ACAD OF INSPECTION & QUARANTINE
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Problems solved by technology

However, the isolation and identification of influenza virus takes a long time and costs high, and needs to be carried out in biosafety laboratories or national designated research institutions, while RT-PCR relies on specific equipment, good laboratory conditions and high-quality techniques Therefore, it is urgent to study and establish a rapid and effective antigen diagnostic technology for new H1N1 influenza virus

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  • Specific synthetic polypeptide for detecting novel H1N1 influenza viruses
  • Specific synthetic polypeptide for detecting novel H1N1 influenza viruses
  • Specific synthetic polypeptide for detecting novel H1N1 influenza viruses

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Embodiment Construction

[0029] The present invention will be described in detail below in conjunction with examples.

[0030] The screening method for specific synthetic polypeptides of the present invention comprises the following steps:

[0031] (1) Bioinformatics selection, synthesis and modification of peptide epitopes

[0032] Search the HA amino acid sequence of influenza virus from the NCBI database, and use the known DNA-STAR and antheprot5.0 analysis to show that the part between 330-350 is a good candidate fragment in terms of antigenicity, hydrophilicity or soluble analysis ; There are a large number of α-helix results downstream of position 400, and they are mainly located in the hydrophobic segment, and the variability of this segment is very low and the sequence is stable. Therefore, it is judged as a possible membrane-penetrating part and this region is discarded. However, after this region (500-520), there is a shorter region with greater variation, and this part has strong antigenic...

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Abstract

The invention discloses a specific synthetic polypeptide for quickly detecting novel H1N1 influenza viruses. The sequence of the polypeptide is CKYVKSTKLRLATGLRNVPS. The specific synthetic polypeptide has the advantages of simple antigen preparation, no biological safety problem, stronger specificity than natural microorganism or parasite protein antigen, low false negative rate and background reaction of detection antibodies and the like. In consideration of the diagnostic method of influenza viruses and the grim situation of prevention and control of influenza around the world currently, the polypeptide, undoubtedly, has relatively high market value and social benefit.

Description

technical field [0001] The invention relates to a synthetic polypeptide for specific detection of novel H1N1 influenza virus. Background technique [0002] Influenza virus, referred to as influenza virus for short, is a type of highly contagious respiratory virus and is also one of the biological terrorism weapons. At present, the methods for detecting the novel H1N1 influenza virus mainly include virus isolation and culture, reverse transcription polymerase chain reaction (RT-PCR), serological diagnosis and antigen detection. However, the isolation and identification of influenza virus takes a long time and costs high, and needs to be carried out in biosafety laboratories or national designated research institutions, while RT-PCR relies on specific equipment, good laboratory conditions and high-quality techniques Therefore, it is urgent to study and establish a rapid and effective antigen diagnostic technology for new H1N1 influenza virus. Contents of the invention [0...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08C07K1/00G01N33/569
Inventor 胡孔新王琳郭雪杨鹏飞平芮巾张丽萍马雪征
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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