Up-conversion luminescent particle detection card for porcine cysticercosis as well as preparation method and application thereof

Abstract

The invention relates to the technical field of biological detection, in particular to an up-conversion luminescent particle detection card for porcine cysticercosis as well as a preparation method and application thereof. The up-conversion luminescent particle detection card comprises a sample pad, a combination pad, an analysis membrane and a water absorption pad. A detection line and a quality control line are arranged on the analysis membrane. The combination pad is coated with goat anti-pig IgG marked by up-conversion luminescent particles, and the detection line is coated with porcine cysticercosis active protein GP50. A nucleotide sequence for coding the cysticercus cellulosae active protein GP50 is as shown in SEQ ID NO: 1. The detection card has the advantages of being simple in antigen preparation, simple, convenient and rapid, easy in result interpretation and the like, does not generate cross reaction with other parasite positive serum, is high in specificity, good in stability, high in practicability and easy to store, can be used for large-scale detection, and has great market prospects.

Description

Technical field [0001] The present invention relates to the field of biological detection, and in particular, to the upper transmissive particle detection card for porcine bladder, the preparation method and application thereof. Background technique [0002] Pig vesicular disease is a human beast who caused by larvae in pig straps in pigs or human parasitic diseases. The disease not only causes huge losses to the pig industry, but also endangered human health, and even cause death. At present, the World Health Organization (WHO) has been listed as "one of the 17 ignored tropical diseases" and determines it as a focus research and control. The "Ten Crison Human Food Severe Parasites", which is published by the United Nations Food and Agriculture Organization (FAO) and the World Health Organization, and the first place in the capsule, and is also one of the key parasitic diseases of the "National Health Department's planning prevention". Diagnosis of porch tape tapkoda depends on i...

AI Technical Summary

Problems solved by technology

The most valuable imaging techniques for cysticercosis are computed tomography (CT) and magnetic resonance imaging (MRI), but the accuracy and sensitivity of these two techniques are highly dependent on cyst location and infection stage, so they have low sensitivity. , high cost and low usability, it is not feasible to use it as a detection of porcine cysticercosis in a timely manner.

In addition, the two detection methods cannot realize the detection of egg invasion to cyst formation stage and human tapeworm disease

Immunological detection methods have the characteristics of good applicability and are widely used. At present, the antigens used in the immunological detection of Taenia solium / cysticercosis are mostly cystic fluid antigens, parasite antigens, excretion / secretion antigens, etc. These antigens are not only derived from Limited, cumbersome to prepare, and severe cross-reactivity with a variety of parasites

Serological methods based on a single recombinant antigen, such as enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunoelectrotransfer blotting (EITB) detection methods, overcome the above shortcomings, but they still have differences in detection with similar diseases (such as echinococcosis, thin neck echinococcosis) has cross-reactivity, and more important defects are the need for expensive instruments and equipment, professional operators, complex data processing, and unsatisfactory sensitivity, which makes the two methods stay at the research stage In the primary stage, it cannot meet the requirements of timely detection

The emergence of colloidal gold immunochromatographic detection method has solved the problems existing in the above detection methods to a certain extent, but its method is difficult to realize multiple analysis and quantitative analysis of samples, and the error of result judgment cannot be avoided by various immunological detection methods. question

In addition, the expression of recombinant antigens selected for diagnosis nowadays, the epitopes of which are easily changed by modification after translation or intricately folded internally, resulting in significantly reduced or lost activity, which affects the effect of detection. The quality and quantity of recombinant antigens is difficult, and the cost is very expensive

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