Application of NOB1 gene in treating brain glioma disease

A technique for brain glioma and glioma, applied in the field of research on the pathogenesis of brain glioma, can solve the problem of unclear specific function of NOB1 gene

Inactive Publication Date: 2011-01-19
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the specific function of the NOB1 gene is still unclear. So far, there are no international literature reports on the application of the NOB1 gene in glioma diseases.

Method used

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  • Application of NOB1 gene in treating brain glioma disease
  • Application of NOB1 gene in treating brain glioma disease
  • Application of NOB1 gene in treating brain glioma disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Materials and methods

[0029] cell culture

[0030] HEK293T cells and glioma cells A172 and U373 were purchased from American Type Culture Collection (ATCC). Using DMEM medium, additionally add 10% fetal bovine serum, 100 U / mL penicillin and 100 μg / mL streptomycin. Cells were cultured in an incubator at 37°C in a humidified atmosphere with 5% CO2.

[0031] RNA extraction

[0032] Human glioma tissue and normal brain tissue were placed in liquid nitrogen immediately after surgery and stored in a -80°C refrigerator until RNA extraction. Total RNA was extracted according to the operation manual of Trizol reagent. Cell lines A172 and U373 were extracted RNA by the same method.

[0033] RNA reverse transcription and Real-time PCR

[0034] Take 2 μg of RNA to synthesize cDNA according to the operation manual of M-MuLV reverse transcriptase. The synthesized cDNA (20 μg) was mixed with SYBR GreenMasterMix and amplified in the CFX96 real-time monitoring system. Each sam...

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PUM

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Abstract

The invention relates to an application of NOB1 gene in treating a brain glioma disease. NOB1 gene can regulate and control cell cycle of human glioma to reduce G1 phase stagnation in the cell cycle caused by NBO1 level; in addition, the NOB1 gene can regulate and control proliferation of human glioma cells to inhibit growth retardation of the glioma cells caused by the NOB1; moreover, the reduction of the NOB1 can prevent occurrence of the human glioma cell tumor. The study finds the NOB1 of the invention is necessary to inhibiting the occurrence of brain glioma in vivo or in vitro; in addition, according to the expression result of the NOB1 gene in the brain glioma tissue, the NOB1 gene provides powerful means for biological targeted therapy of the glioma in the future.

Description

technical field [0001] The invention relates to the research field of the pathogenesis of brain glioma, and in particular relates to an application of NOB1 gene in brain glioma disease. Background technique [0002] Glioma is a highly invasive and lethal solid tumor derived from the supporting cells of the central nervous system, and can be divided into astrocytoma, oligodendroglioma, and oligoastrocytoma. Astrocytoma accounts for 80-85% of gliomas, and can be divided into grade I to grade IV according to pathology. Grade IV glioma, also known as glioblastoma, is the most common pathological type of glioma with a poor prognosis. Although researchers have done a lot of work in the treatment of glioma, the survival time of some patients with glioblastoma is still less than 15 months. Although there are a lot of studies on the etiology and prognosis of glioma, the research on the gene level of glioma is still in its infancy. [0003] In eukaryotic cells, proteasome 26S is a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N5/09A61K49/00
Inventor 陈菊祥卢亦成孙克华周劲旭
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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