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Method for domesticating cylindrospermum and application thereof

A technology for Cylindrosporium and algae species, applied in chemical instruments and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of lack of soil nutrients, large material and financial resources, high treatment costs, etc. The effect of reducing treatment cost, increasing fertility and improving planting effect

Inactive Publication Date: 2013-02-27
北京芳能科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

) Among the existing main methods for controlling saline-alkali land, the water washing and salt discharge adopted by the physical method are likely to cause the loss of soil nutrients, and require large material and financial resources, while the fertilization or acid-base balance adopted by the chemical method is likely to cause soil compaction and Excessive governance costs
Although physical and chemical methods can temporarily change the salinity of the soil, they cannot essentially change the soil fertility of the original saline-alkali land.

Method used

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  • Method for domesticating cylindrospermum and application thereof
  • Method for domesticating cylindrospermum and application thereof
  • Method for domesticating cylindrospermum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Treatment of saline-alkali areas around Canglangqu in Binhai New Area, Tianjin

[0058] Step 1: 1. Prepare the FN30 medium (basic medium) for cultivating Cylindrospora:

[0059] Ammonium nitrate 1.5g / L Sodium acetate 0.015g / L

[0060] Potassium dihydrogen phosphate 0.03g / L Calcium chloride 0.03g / L

[0061] Magnesium sulfate 0.02g / L Sodium chloride 0.7g / L

[0062] Urea 0.05g / L Trace elements 1.5ml / L

[0063] Wherein the trace element preparation method is:

[0064] Ferric Chloride 5.8mg Sodium Molybdate 0.34mg

[0065] Manganese Chloride 2.6mg Disodium EDTA 75mg

[0066] Zinc sulfate 0.72mg Cobalt chloride 0.11mg

[0067] Add distilled water to make up to 1L

[0068] The culture conditions are: temperature 30°C, light 15000 lux, pH: 8.5, after 7 days of culture, the dry cell weight of Cylindrosporium can reach up to 1.2g / L. ( figure 2 )

[0069] 2. The formula of Allen medium is as follows:

[0070] Sodium nitrate 1.5g / L Magnesium sulfate 0.0375g / L

[0071] ...

Embodiment 2

[0099] Treatment of saline-alkali areas around Canglangqu in Binhai New Area, Tianjin

[0100] Step 1: Prepare the medium (basic medium) for cultivating Cylindrospora:

[0101] Ammonium nitrate 2.1g / L Sodium acetate 0.02g / L

[0102] Potassium dihydrogen phosphate 0.06g / L Calcium chloride 0.01g / L

[0103] Magnesium sulfate 0.03g / L Sodium chloride 0.5g / L

[0104] Urea 0.03g / L Trace elements 0.5ml / L

[0105] The culture conditions are: temperature 28°C, light 10000 lux, pH: 8.0, growth cycle 7 days.

[0106] Step 2: Adaptive domestication of Cylindrosporium according to the use of saline-alkali land:

[0107] (1). Salinity tolerance acclimatization:

[0108] Ammonium nitrate 2.1g / L Sodium acetate 0.02g / L

[0109] Potassium dihydrogen phosphate 0.06g / L Calcium chloride 0.01g / L

[0110] Magnesium sulfate 0.03g / L Urea 0.05g / L

[0111] Trace elements 0.5ml / L Sodium chloride gradually increased from 2g / L to 5g / L

[0112] After being acclimatized with tolerance to salinity, se...

Embodiment 3

[0128] Treatment of saline-alkali areas around Canglangqu in Binhai New Area, Tianjin

[0129] Step 1: Prepare the medium (basic medium) for cultivating Cylindrospora:

[0130] Ammonium nitrate 3.0g / L Sodium acetate 0.01g / L

[0131] Potassium dihydrogen phosphate 0.07g / L Calcium chloride 0.02g / L

[0132] Magnesium Sulfate 0.01g / L Sodium Chloride 1.0g / L

[0133] Urea 0.06g / L Trace elements 1.0ml / L

[0134] The culture conditions are: temperature 29°C, light 18000 lux, pH: 8.5, growth cycle 7 days.

[0135] Step 2: Adaptive domestication of Cylindrosporium according to the use of saline-alkali land:

[0136] (1). Salinity tolerance acclimatization:

[0137] Ammonium nitrate 3.0g / L Sodium acetate 0.01g / L

[0138] Potassium dihydrogen phosphate 0.07g / L Calcium chloride 0.02g / L

[0139] Magnesium sulfate 0.01g / L Urea 0.06g / L

[0140] Trace elements 1.0ml / L Sodium chloride gradually increased from 2g / L to 6g / L

[0141] After being acclimatized with tolerance to salinity, se...

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Abstract

The invention discloses a method for domesticating cylindrospermum and application thereof. The method comprises the following steps of: preparing a specific culture medium; carrying out salinity tolerance domestication on the screened algaes; reducing the domestication of nitrogen and phosphorus contents; adapting to the domestication on the quality of water to be treated and the like; introducing the algaes into saline and alkaline land after gradual amplifying culture; reproducing the algaes in a large mount by using photosynthesis; absorbing and fixing nitrogen and phosphorus elements in the atmosphere and water sources; after reproduction, draining algaes breeding water; and depositing the algaes into soil, wherein the algaes contain a large amount of nitrogen, phosphor and other elements. After the algaes are primarily treated, the nitrogen in the soil is increased by 18-20 percent, the content of phosphor is increased by 11-13 percent and the content of all salts is reduced by 12-15 percent. The invention not only prevents nutrient elements by a washing method from being lost, but also prevents applied fertilizer from hardening soil and secondary pollution to the water source and the environment; and after multiple treatments, the soil fertility can be gradually enhanced, the soil restoration can be accelerated and the finally the aim of improving the ecological environment can be achieved.

Description

technical field [0001] The invention discloses a method for improving saline-alkali land and increasing soil fertility by using nitrogen-fixing blue-green algae, in particular to a method for treating saline-alkali land by using Cylindrosporium algae. Background technique [0002] Saline-alkali lands are widely distributed around the world, covering more than 30 countries on six continents. It is mainly distributed in India, Pakistan, Russia, South Africa and other countries, with a total area of ​​about 956 million hectares. Among them, India and Pakistan have 8.09 million hectares, and Russia has 7.5 million hectares. The area of ​​saline-alkali land in the western United States accounts for about half of the irrigated area, and the output of saline-alkali is reduced by more than 25% every year. Land salinization is an important part of land desertification, which seriously threatens the local agricultural and animal husbandry production, the prosperity and development o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C09K17/00C12R1/89C09K109/00
Inventor 单东杰李涛
Owner 北京芳能科技有限公司
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