Method for domesticating cylindrospermum and application thereof
A technology for Cylindrosporium and algae species, applied in chemical instruments and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of lack of soil nutrients, large material and financial resources, high treatment costs, etc. The effect of reducing treatment cost, increasing fertility and improving planting effect
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Embodiment 1
[0057] Treatment of saline-alkali areas around Canglangqu in Binhai New Area, Tianjin
[0058] Step 1: 1. Prepare the FN30 medium (basic medium) for cultivating Cylindrospora:
[0059] Ammonium nitrate 1.5g / L Sodium acetate 0.015g / L
[0060] Potassium dihydrogen phosphate 0.03g / L Calcium chloride 0.03g / L
[0061] Magnesium sulfate 0.02g / L Sodium chloride 0.7g / L
[0062] Urea 0.05g / L Trace elements 1.5ml / L
[0063] Wherein the trace element preparation method is:
[0064] Ferric Chloride 5.8mg Sodium Molybdate 0.34mg
[0065] Manganese Chloride 2.6mg Disodium EDTA 75mg
[0066] Zinc sulfate 0.72mg Cobalt chloride 0.11mg
[0067] Add distilled water to make up to 1L
[0068] The culture conditions are: temperature 30°C, light 15000 lux, pH: 8.5, after 7 days of culture, the dry cell weight of Cylindrosporium can reach up to 1.2g / L. ( figure 2 )
[0069] 2. The formula of Allen medium is as follows:
[0070] Sodium nitrate 1.5g / L Magnesium sulfate 0.0375g / L
[0071] ...
Embodiment 2
[0099] Treatment of saline-alkali areas around Canglangqu in Binhai New Area, Tianjin
[0100] Step 1: Prepare the medium (basic medium) for cultivating Cylindrospora:
[0101] Ammonium nitrate 2.1g / L Sodium acetate 0.02g / L
[0102] Potassium dihydrogen phosphate 0.06g / L Calcium chloride 0.01g / L
[0103] Magnesium sulfate 0.03g / L Sodium chloride 0.5g / L
[0104] Urea 0.03g / L Trace elements 0.5ml / L
[0105] The culture conditions are: temperature 28°C, light 10000 lux, pH: 8.0, growth cycle 7 days.
[0106] Step 2: Adaptive domestication of Cylindrosporium according to the use of saline-alkali land:
[0107] (1). Salinity tolerance acclimatization:
[0108] Ammonium nitrate 2.1g / L Sodium acetate 0.02g / L
[0109] Potassium dihydrogen phosphate 0.06g / L Calcium chloride 0.01g / L
[0110] Magnesium sulfate 0.03g / L Urea 0.05g / L
[0111] Trace elements 0.5ml / L Sodium chloride gradually increased from 2g / L to 5g / L
[0112] After being acclimatized with tolerance to salinity, se...
Embodiment 3
[0128] Treatment of saline-alkali areas around Canglangqu in Binhai New Area, Tianjin
[0129] Step 1: Prepare the medium (basic medium) for cultivating Cylindrospora:
[0130] Ammonium nitrate 3.0g / L Sodium acetate 0.01g / L
[0131] Potassium dihydrogen phosphate 0.07g / L Calcium chloride 0.02g / L
[0132] Magnesium Sulfate 0.01g / L Sodium Chloride 1.0g / L
[0133] Urea 0.06g / L Trace elements 1.0ml / L
[0134] The culture conditions are: temperature 29°C, light 18000 lux, pH: 8.5, growth cycle 7 days.
[0135] Step 2: Adaptive domestication of Cylindrosporium according to the use of saline-alkali land:
[0136] (1). Salinity tolerance acclimatization:
[0137] Ammonium nitrate 3.0g / L Sodium acetate 0.01g / L
[0138] Potassium dihydrogen phosphate 0.07g / L Calcium chloride 0.02g / L
[0139] Magnesium sulfate 0.01g / L Urea 0.06g / L
[0140] Trace elements 1.0ml / L Sodium chloride gradually increased from 2g / L to 6g / L
[0141] After being acclimatized with tolerance to salinity, se...
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