Method for culturing directly differentiated tissues of cotton true leaf and special culture medium

Abstract

The invention discloses a method for culturing directly differentiated tissues of a cotton true leaf and a special culture medium. The special culture medium provided by the invention is a culture medium which is used when embryogenic callus is directly differentiated from a cotton leaf; and the culture medium is obtained by adding KT, 6-BA, 2,4-D, indole acetic acid (IAA), glucose and gel into marek's disease 1 (MSB1) basic culture solution, wherein the final concentration of the KT, the 6-BA, the 2,4-D, the IAA, the glucose and the Gel rite is 0.005 to 0.15mg / L, 0.005 to 0.15mg / L, 0.0001 to 0.001mg / L, 0.001 to 0.02mg / L, 25 to 35g / L and 1.8 to 2.5g / L respectively. By the method, a tissue culture system is established by taking leaves of field cotton plants as explants; and finally, a stable and high-efficiency tissue culture system of the directly induced embryogenic callus of the leaf is established.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a special culture medium for a field cotton plant true leaf tissue culture method. Background technique [0002] In cotton tissue culture, somatic embryogenesis and plant regeneration can be divided into four steps: explant dedifferentiation induces callus, callus redifferentiates into embryogenic callus, embryogenic callus cultures somatic cells Embryos and somatic embryos germinated to obtain plant regeneration. It is relatively easy to grow callus from explants by dedifferentiation, and there is no report of genotype restriction. The induction and differentiation of embryogenic callus from callus is the bottleneck of cotton tissue culture, and the reports limited by genotype focus on the induction stage of embryogenic callus. [0003] Genotype restriction is firstly manifested in the different somatic embryogenesis and plant regeneration abilities of different cotton spec...

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Problems solved by technology

The hypocotyl is the easiest, followed by the mesocotyl and epicotyl, the cotyledons are poor, and the leaves and stems are the worst. In recent years, hairy roots can be regenerated, but there is no comparison of the degree of difficulty (Jiao Gaili et al., 2002); Zhang Hai et al. (2002) reported the in vitro culture and plant regeneration of cotton cotyledons, but the regeneration frequency was not high.

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