Application of palmate pinellia tuber protein in treatment of cervical carcinoma and preparation thereof

A technology of pinellia palmatum and cervical cancer, which is applied in the field of pharmaceutical composition for treating cervical cancer, application of Pinellia palmatum protein in treating cervical cancer and its preparation, and can solve problems such as side effects

Inactive Publication Date: 2011-06-29
CHONGQING PHARMA RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] For cervical cancer, there is currently no effective therapeutic drug, but radiotherapy, chemotherapy, and surgery are still the main treatments. Merck and GlaxoSmithKline have designed and developed Garda...

Method used

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  • Application of palmate pinellia tuber protein in treatment of cervical carcinoma and preparation thereof
  • Application of palmate pinellia tuber protein in treatment of cervical carcinoma and preparation thereof
  • Application of palmate pinellia tuber protein in treatment of cervical carcinoma and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 Preparation of Pinellia palmata protein

[0061] Instruments: refrigerator (Rongsheng BCD-271); tissue homogenizer; CP224S analytical balance (Sartorius Instrument Co., Ltd.); 5804R high-speed refrigerated centrifuge (Eppendorf Germany).

[0062] Take 100g of the washed Pinellia palmata medicinal material, use 400mL of PBS solution with pH 7.4 in a tissue homogenizer, soak in 200mL of the same PBS solution at 4°C for 12h, and centrifuge at 6000rpm / min for 15 minutes to obtain 605mL of brown supernatant, add Ammonium sulfate until the concentration of ammonium sulfate reaches 30%, carry out precipitation, stand in the refrigerator at 4°C for 19 hours, centrifuge at 6000rpm / min at low temperature for 15min, discard the supernatant, and dissolve the solid obtained from the above 30% ammonium sulfate in 2ml of PBS The solution is passed through a desalting chromatography column, desalted with PBS solution, the flow rate is 10ml / min, the detection wavelength is 280...

Embodiment 2

[0063] Example 2 Preparation of Pinellia palmata protein

[0064]Instruments: refrigerator (Rongsheng BCD-271); tissue homogenizer; CP224S analytical balance (Sartorius Instrument Co., Ltd.); 5804R high-speed refrigerated centrifuge (eppendorf Germany).

[0065] Take 100g of the washed Pinellia palmata medicinal material, use 800mL of PBS solution with pH 7.4 in a tissue homogenizer, soak in 100mL of the same PBS solution at 2°C for 9h, and centrifuge at 3000rpm / min for 30 minutes to obtain 910mL of brown supernatant, add Ammonium sulfate until the concentration of ammonium sulfate reaches 65%, carry out precipitation, stand in the refrigerator at 2°C for 16 hours, centrifuge at 3000rpm / min at low temperature for 30min, discard the supernatant, and dissolve the solid matter obtained from the above 65% ammonium sulfate in 2ml of PBS The solution is passed through a desalting chromatography column, desalted with PBS solution, the flow rate is 10ml / min, the detection wavelength i...

Embodiment 3

[0066] Example 3 Preparation of Pinellia palmata protein

[0067] Instruments: refrigerator (Rongsheng BCD-271); tissue homogenizer; CP224S analytical balance (Sartorius Instrument Co., Ltd.); 5804R high-speed refrigerated centrifuge (Eppendorf Germany).

[0068] Take 100g of the washed Pinellia palmata medicinal material, use 300mL of PBS solution with pH 7.4 in a tissue homogenizer, soak in 600mL of the same PBS solution at 5°C for 15h, and centrifuge at 7000rpm / min for 10 minutes to obtain 907mL of brown supernatant, add Ammonium sulfate until the concentration of ammonium sulfate reaches 55%, carry out precipitation, put it in the refrigerator at 5°C for 22 hours, centrifuge at 7000rpm / min at low temperature for 10min, discard the supernatant, and dissolve the solid obtained from the above 55% ammonium sulfate in 2ml of PBS The solution is passed through a desalting chromatography column, desalted with PBS solution, the flow rate is 10ml / min, the detection wavelength is 28...

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Abstract

The invention relates to application of palmate pinellia tuber protein in the treatment of cervical carcinoma and a preparation thereof. The palmate pinellia tuber protein is total protein, and is prepared by extracting from tubers of palmate pinellia tuber. The palmate pinellia tuber protein has cervical carcinoma resistant activity, and the activity is higher than that of 5-fluorouracil obviously. The invention also relates to a palmate pinellia tuber protein-containing medicinal composition for treating cervical carcinoma.

Description

technical field [0001] The technology relates to the field of traditional Chinese medicine pharmacy, and relates to the use of pinellia palmata protein in treating cervical cancer and its preparation. The palm leaf Pinellia protein is a total protein and has anti-cervical cancer activity. The invention also relates to a pharmaceutical composition for treating cervical cancer containing palm leaf pinellia protein. Background technique [0002] Cervical cancer is a malignant tumor that usually occurs at the junction of the squamous epithelial cells of the cervicovaginal or transition zone and the columnar epithelial cells of the endocervical endometrium. It accounts for more than 50% of malignant tumors in the female reproductive system, and its case fatality rate ranks first in female malignant tumors. It is one of the most common malignant tumors in women. According to the report of the World Health Organization, 459,000 cases of cervical cancer are newly discovered every ...

Claims

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Application Information

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IPC IPC(8): A61K36/8888A61K9/08A61K9/19A61K9/00A61P35/00A61K125/00
Inventor 丁彦吉潘正张涛曾凡新邓杰钟怡
Owner CHONGQING PHARMA RES INST
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