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A method for producing turbot immunoglobulin

An immunoglobulin and turbot technology, applied in the direction of anti-animal/human immunoglobulin, etc., can solve the problems of insufficient specific immunoglobulin and lack of effective antigen, etc. obtained effect

Inactive Publication Date: 2011-12-07
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for producing turbot immunoglobulin, which can effectively solve the problem of lack of effective antigen and insufficient quantity of specific immunoglobulin in the immune research of turbot

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  • A method for producing turbot immunoglobulin
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  • A method for producing turbot immunoglobulin

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Embodiment Construction

[0016] The bovine serum albumin (BSA) adopted in the present invention, also known as the fifth component, is a simple globulin in bovine serum, consisting of 583 amino acid residues, with a molecular weight of 66.430kDa and an isoelectric point of 4.7. BSA is widely used in biochemical experiments, for example, as a blocking solution in western blot; BSA is also a commonly used carrier protein, and cross-linking it to haptens and other weak antigens can make them more effective in antibody production Immunogenicity. In the existing immunological research, there is no report on the application of BSA directly as an antigen. The invention introduces BSA into fish immunology research, opens up a new way to study immunoglobulin, thereby overcoming the shortcomings of previous methods.

[0017] Concrete method of the present invention is as follows:

[0018] 1) The first immunization: mix and emulsify BSA and Freund's complete adjuvant in equal proportions to make a vaccine, and...

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Abstract

The invention relates to a method for producing turbot immunoglobulin, which is characterized in that the turbot is immunized by using bovine serum albumin BSA as an antigen; specifically, after three immunization operations, after the first immunization The anti-BSA immunoglobulin of turbot was obtained by affinity chromatography within 8 to 10 weeks. The invention overcomes the conventional usage of BSA in the field of biochemistry, and uses it as an antigen to immunize turbot through a specific immunization procedure, so as to obtain high titer anti-BSA specific antibody. The method of the invention solves the long-standing problem of lack of sufficient specific immunoglobulin in turbot immunization research. The BSA used at the same time has the advantages of simple structure, single component, not easy to interfere with immune results, and easy to obtain. BSA can be established as a model protein in aquatic animals, and a research model based on BSA can be formed.

Description

technical field [0001] The invention belongs to the technical field of aquatic animal immunology research, and in particular relates to a method for producing turbot immunoglobulin. Background technique [0002] The basic research on the whole immune system of fish and the rules of immune response depends on the research on immunoglobulin, which is the main mediator of fish immune response. Therefore, the research on immunoglobulin is also the focus and hotspot of fish immunology research. How to make the fish immune response and how to obtain high-purity immunoglobulin is the key to the research. In the study of turbot immunization, in the past, it was mainly used to immunize the fish body with a vaccine made of pathogens to produce specific antibodies, and then to purify the antibodies by saturated ammonium sulfate precipitation, gel filtration and other techniques to study the immunoglobulin. characteristic. However, the activity, purity and quantity of immunoglobulins...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/18
Inventor 王蔚芳李青梅甘玲玲张改平雷霁霖
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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